Mouse anti-Human Granzyme H Monoclonal Antibody | anti-GZMH antibody

Anti-Granzyme H Antibody, Mouse Monoclonal

Cat. Num. AAA8102403

• Gene Names: GZMH; CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
• Reactivity: Human
• Applications: ELISA
• Purity: Protein A
• Synonyms: Granzyme H; Monoclonal Antibody; Anti-Granzyme H Antibody; Mouse Monoclonal; Granzyme H/GZMH Antibody; Mouse MAb; granzyme H; Anti-CCP-X Antibody; Anti-CGL-2 Antibody; Anti-CSP-C Antibody; Anti-CTLA1 Antibody; Anti-CTSGL2 Antibody; Anti-GZMH Antibody; anti-GZMH antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: 3G10F4
• Specificity: Human Granzyme H/GZMH.; No cross-reactivity in ELISA with Human Granzyme B, Human cell lysate (293 cell line)
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GZMH antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000
• Immunogen: Recombinant Human Granzyme H/GZMH protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Granzyme H/GZMH (rh Granzyme H/GZMH). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-GZMH antibody

Granzymes are key components of the immune response that play important roles in eliminating host cells infected by intracellular pathogens. Several granzymes are potent inducers of cell death. A total of eight granzymes (A-G and M) have been identified in the mouse, but only five are known in humans (A, B, H, M, and granzyme 3), and granzyme H appears to be specifically human. Human granzyme H is a neutral serine protease that is expressed predominantly in the lymphokine-activated killer (LAK)/natural?killer (NK) compartment of the immune system. In adenovirus-infected cells in which granzyme B (gzmB) and downstream apoptosis pathways are inhibited, granzyme H directly cleaves the adenovirus DNA-binding protein (DBP), a viral component required for viral DNA replication. This virus demonstrated that gzmH directly induces an important decay in viral DNA replication. Interestingly, gzmH also cleaves the adenovirus 1K assembly protein, a major inhibitor of gzmB, and relieves gzmB inhibition. Granzyme H has a very high amino acid identity (>9%) with many portions of the granzyme B sequence, particularly near the amino terminus of the molecule despite performing a distinct enzymic function.

References

Meier M., et al., (1990), Cloning of a gene that encodes a new member of the human cytotoxic cell protease family. Biochemistry 29:4042-4049.Haddad P., et al., (1991), Structure and evolutionary origin of the human granzyme H gene.Int. Immunol. 3:57-66.Klein J.L., et al., (1990), Characterization of a novel, human cytotoxic lymphocyte-specific serine protease cDNA clone (CSP-C).Tissue Antigens 35:220-228.

NCBI and Uniprot Product Information

• NCBI GI #: 15529990
• NCBI GeneID: 2999
• NCBI Accession #: NP_219491.1
• NCBI GenBank Nucleotide #: NM_033423.4
• UniProt Accession #: P20718
• Molecular Weight: 27,315 Da
• NCBI Official Full Name: granzyme H isoform 1
• NCBI Official Synonym Full Names: granzyme H (cathepsin G-like 2, protein h-CCPX)
• NCBI Official Symbol: GZMH
• NCBI Official Synonym Symbols: CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
• NCBI Protein Information: granzyme H; cytotoxin serine protease-C; cytotoxic T-lymphocyte proteinase; cytotoxic T-lymphocyte-associated serine esterase 1
• UniProt Protein Name: Granzyme H
• Protein Family: Granzyme
• UniProt Gene Name: GZMH
• UniProt Synonym Gene Names: CGL2; CTSGL2; CTSGL2; CSP-C
• UniProt Entry Name: GRAH_HUMAN

NCBI Description

The protein encoded by this gene is a member of the granzyme family. Members of this family are highly conserved serine proteases that eliminate transformed cells and virus-infected cells. This protein, which has chymotrypsin-like activity, has a preference for bulky aromatic amino acids at the P1 position and for acidic residues at the P3' and P4' positions. This protein is reported to be constitutively expressed in NK cells and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

GZMF: Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: EC 3.4.21.-; Protease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: serine-type endopeptidase activity

Biological Process: apoptosis; cytolysis; immune response; protein processing; proteolysis