Granzyme H Recombinant Protein | GZMH recombinant protein

Granzyme H Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120219

• Gene Names: GZMH; CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Granzyme H; Granzyme H Protein; Human; Recombinant (His Tag); Human Granzyme H Protein (His Tag); CCP-X Protein; CGL-2 Protein; CSP-C Protein; CTLA1 Protein; CTSGL2 Protein; GZMH Protein; granzyme H; GZMH recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu246

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human Granzyme H (NP_219491.1)(Met 1-Leu 246) with a C-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GZMH recombinant protein

Background: Granzymes are key components of the immune response that play important roles in eliminating host cells infected by intracellular pathogens. Several granzymes are potent inducers of cell death. A total of eight granzymes (A-G and M) have been identified in the mouse, but only five are known in humans (A, B, H, M, and granzyme 3), and granzyme H appears to be specifically human. Human granzyme H is a neutral serine protease that is expressed predominantly in the lymphokine-activated killer (LAK)/naturalkiller (NK) compartment of the immune system. In adenovirus-infected cells in which granzyme B (gzmB) and downstream apoptosis pathways are inhibited, granzyme H directly cleaves the adenovirus DNA-binding protein (DBP), a viral component required for viral DNA replication. This virus demonstrated that gzmH directly induces an important decay in viral DNA replication. Interestingly, gzmH also cleaves the adenovirus 1K assembly protein, a major inhibitor of gzmB, and relieves gzmB inhibition. Granzyme H has a very high amino acid identity (>9%) with many portions of the granzyme B sequence, particularly near the amino terminus of the molecule despite performing a distinct enzymic function.

References

Meier M., et al.,(1990), Cloning of a gene that encodes a new member of the human cytotoxic cell protease family. Biochemistry 29:4042-4049.Haddad P., et al., (1991), Structure and evolutionary origin of the human granzyme H gene.Int. Immunol. 3:57-66.Klein J.L., et al.,(1990), Characterization of a novel, human cytotoxic lymphocyte-specific serine protease cDNA clone (CSP-C).Tissue Antigens 35:220-228.

NCBI and Uniprot Product Information

• NCBI GI #: 15529990
• NCBI GeneID: 2999
• NCBI Accession #: NP_219491.1
• NCBI GenBank Nucleotide #: NM_033423.4
• UniProt Accession #: P20718
• Molecular Weight: 27,315 Da
• NCBI Official Full Name: granzyme H isoform 1
• NCBI Official Synonym Full Names: granzyme H (cathepsin G-like 2, protein h-CCPX)
• NCBI Official Symbol: GZMH
• NCBI Official Synonym Symbols: CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
• NCBI Protein Information: granzyme H; cytotoxin serine protease-C; cytotoxic T-lymphocyte proteinase; cytotoxic T-lymphocyte-associated serine esterase 1
• UniProt Protein Name: Granzyme H
• Protein Family: Granzyme
• UniProt Gene Name: GZMH
• UniProt Synonym Gene Names: CGL2; CTSGL2; CTSGL2; CSP-C
• UniProt Entry Name: GRAH_HUMAN

NCBI Description

The protein encoded by this gene is a member of the granzyme family. Members of this family are highly conserved serine proteases that eliminate transformed cells and virus-infected cells. This protein, which has chymotrypsin-like activity, has a preference for bulky aromatic amino acids at the P1 position and for acidic residues at the P3' and P4' positions. This protein is reported to be constitutively expressed in NK cells and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

GZMF: Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: EC 3.4.21.-; Protease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: serine-type endopeptidase activity

Biological Process: apoptosis; cytolysis; immune response; protein processing; proteolysis