Rabbit ATF2 Polyclonal Antibody | anti-ATF2 antibody

ATF2 (Phospho-Thr71 or 53)

Cat. Num. AAA191043

• Gene Names: ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
• Applications: Immunoblot, Western Blot, Immunohistochemistry
• Synonyms: ATF2; Polyclonal Antibody; ATF2 (Phospho-Thr71 or 53); anti-ATF2 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous human, mouse, and rat ATF2 only when phosphorylated at threonine 71 or 53.
• Form/Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
• Sequence Length: 505

• Applicable Applications for anti-ATF2 antibody: Immunoblotting (IB), Immunohistochemistry (IHC)
• Application Notes: Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~65-75kDa is detected.; Immunohistochemistry: use at dilution of 1:50- 1:100.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Peptide sequence that includes phosphorylation sites of threonine 71 or 53 (T-P-T(p)- P-T) derived from human ATF2 and conjugated to KLH.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Can be stored at 4 degree C for short-term use.

Immunohistochemistry (IHC)

Testing Data

Related Product Information for anti-ATF2 antibody

ATF2 is a transcription factor that is a member of the leucine zipper family of DNA binding protein that can perform multiple functions. It can bind to the cAMP-responsive element (CRE), and it can form a homodimer or a heterodimer with c-Jun and stimulate CRE-dependent transcription. ATF2 is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro. ATF2 may also be involved in a cell's DNA damage response independent of its role in transcriptional regulation.

NCBI and Uniprot Product Information

• NCBI GI #: 22538422
• NCBI GeneID: 1386
• NCBI Accession #: NP_001871.2
• NCBI GenBank Nucleotide #: NM_001880.3
• UniProt Accession #: P15336; Q13000; Q3B7B7; Q4ZFU9; Q53RY2; Q8TAR1; A1L3Z2; A4D7U4; A4D7U5; A4D7V1; D3DPE9; G8JLM5
• Molecular Weight: 15,409 Da
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-2 isoform 1
• NCBI Official Synonym Full Names: activating transcription factor 2
• NCBI Official Symbol: ATF2
• NCBI Official Synonym Symbols: HB16; CREB2; TREB7; CREB-2; CRE-BP1
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-2
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-2
• Protein Family: Alcohol O-acetyltransferase
• UniProt Gene Name: ATF2
• UniProt Synonym Gene Names: CREB2; CREBP1; cAMP-dependent transcription factor ATF-2; CREB-2; cAMP-responsive element-binding protein 2

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]

Uniprot Description

ATF-2: a transcription factor that is a member of the leucine zipper family. Binds to the cAMP-responsive element (CRE). Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Also possesses histone acetyltransferase (HAT) activity that specifically acetylates histones H2B and H4 in vitro.

Protein type: C2H2-type zinc finger protein; EC 2.3.1.48; Transcription factor

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: cytoplasm; mitochondrial outer membrane; nucleoplasm; nucleus

Molecular Function: cAMP response element binding protein binding; histone acetyltransferase activity; protein binding; protein kinase binding; RNA polymerase II transcription factor activity, enhancer binding; transcription coactivator activity; transcription factor activity

Biological Process: intra-S DNA damage checkpoint; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription factor activity; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to osmotic stress