Mouse anti-Human CD9 Antibody | anti-CD9 antibody

Mouse anti-Human CD9, Purified mA

Cat. Num. AAA8505638

• Gene Names: CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD9; Mouse anti-Human CD9; Purified mA; anti-CD9 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: 4AHI9a
• Form/Format: Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
• Sequence Length: 228

• Applicable Applications for anti-CD9 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Experimental Methods: 1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube; ; 2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature; ; 3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×); ; 4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant; ; 5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant; ; 6.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.; 7.Repeat step 5.; 8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).; [PBS wash buffer: PBS +1% FBS +0.1% NaN3]; [Cell fixation: 2% formaldehyde solution]
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

Testing Data

Related Product Information for anti-CD9 antibody

4AHI9a reacts with CD9 (p24), a member of the tetraspan (TM4SF) family with 24 kDa MW, expressed on platelets and weakly on B-cells. It also expressed on eosinophils, basophils, endothelial and epithelial cells. CD9 antigen modulates cell adhesion, migration and platelet activation. GM1CD9 triggers platelet activation resulted in platelet aggregation, but it is blocked by anti-Fc receptor antibody CD32. This clone is cross reactive with non-human primate.

References

1. Lanza F et al. (1991) J biol Chem 266:10638. 2. Wright MD and Tomlinson MG (1994) Immunology Today 15:58. 3. Tai XG et al. (1996). J Exp Med 184:753. 4. Oritani K et al. (1996). Blood 87: 2252.

NCBI and Uniprot Product Information

• NCBI GI #: 4502693
• NCBI GeneID: 928
• NCBI Accession #: NP_001760.1
• NCBI GenBank Nucleotide #: NM_001769.3
• UniProt Accession #: P21926; Q5J7W6; Q96ES4; D3DUQ9
• Molecular Weight: 25,416 Da
• NCBI Official Full Name: CD9 antigen isoform 1
• NCBI Official Synonym Full Names: CD9 molecule
• NCBI Official Symbol: CD9
• NCBI Official Synonym Symbols: MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
• NCBI Protein Information: CD9 antigen
• UniProt Protein Name: CD9 antigen
• Protein Family: CD9 antigen
• UniProt Gene Name: CD9
• UniProt Synonym Gene Names: MIC3; TSPAN29; MRP-1; Tspan-29

NCBI Description

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

Uniprot Description

Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.