Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Cerebrum lysate; Lane2: Rat Heart lysate; Lane3: HepG2 cell lysatePrimary Ab: 2ug/ml Mouse Anti-Mouse PEDF AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody)
Mouse anti-Mouse Pigment Epithelium Derived Factor (PEDF) Monoclonal Antibody | anti-PEDF antibody
Monoclonal Antibody to Pigment Epithelium Derived Factor (PEDF)
Gene Names
Serpinf1; Pedf; Sdf3; EPC-1; Pedfl; AI195227
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Pigment Epithelium Derived Factor (PEDF); Monoclonal Antibody; Monoclonal Antibody to Pigment Epithelium Derived Factor (PEDF); Cytokine; Neuroscience; anti-PEDF antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Clone Number
C3
Specificity
The antibody is a mouse monoclonal antibody raised against PEDF. It has been selected for its ability to recognize PEDF in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence
Ser19~Thr417
Sequence Length
417
Applicable Applications for anti-PEDF antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:500-5000
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
Conjugation
None
Cross Reactivity
Mouse
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Cerebrum lysate; Lane2: Rat Heart lysate; Lane3: HepG2 cell lysatePrimary Ab: 2ug/ml Mouse Anti-Mouse PEDF AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody)
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Cerebrum lysate; Lane2: Rat Heart lysate; Lane3: HepG2 cell lysatePrimary Ab: 2ug/ml Mouse Anti-Mouse PEDF AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pigment epithelium-derived factor
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade F, member 1
NCBI Official Symbol
Serpinf1
NCBI Official Synonym Symbols
Pedf; Sdf3; EPC-1; Pedfl; AI195227
NCBI Protein Information
pigment epithelium-derived factor
UniProt Protein Name
Pigment epithelium-derived factor
UniProt Gene Name
Serpinf1
UniProt Synonym Gene Names
Pedf; Sdf3; PEDF; SDF-3
Uniprot Description
Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Research Articles on PEDF
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Product Notes
The PEDF serpinf1 (Catalog #AAA2111261) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Pigment Epithelium Derived Factor (PEDF) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Pigment Epithelium Derived Factor (PEDF) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:500-5000 IHC: 5-20ug/mL; 1:50-200 ICC: 5-20ug/mL; 1:50-200. Researchers should empirically determine the suitability of the PEDF serpinf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ser19~Thr4 17. It is sometimes possible for the material contained within the vial of "Pigment Epithelium Derived Factor (PEDF), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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