Human Hepatocyte Growth Factor Receptor (HGFR) ELISA Kit | HGFR elisa kit

Human Hepatocyte Growth Factor Receptor (HGFR) ELISA Kit

Cat. Num. AAA2704263

• Gene Names: MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
• Reactivity: Human
• Synonyms: Hepatocyte Growth Factor Receptor (HGFR); Human Hepatocyte Growth Factor Receptor (HGFR) ELISA Kit; CMET; RCCP2; C-Met; Met Proto-Oncogene; Mesenchymal-Epithelial Transition Factor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; Tyrosine-protein kinase Met; HGFR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hepatocyte Growth Factor Receptor (HGFR).; No significant cross-reactivity or interference between Hepatocyte Growth Factor Receptor (HGFR) and analogues was observed.
• Sequence Length: 764

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 125-8000pg/mL
• Sensitivity: < 49pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Growth Factor Receptor (HGFR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Growth Factor Receptor (HGFR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HGFR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Growth Factor Receptor (HGFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Growth Factor Receptor (HGFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Growth Factor Receptor (HGFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Growth Factor Receptor (HGFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 42741655
• NCBI GeneID: 4233
• NCBI Accession #: NP_000236.2
• NCBI GenBank Nucleotide #: NM_000245.3
• UniProt Accession #: P08581; O60366; Q12875; Q9UDX7; Q9UPL8; A1L467; B5A932; E7EQ94
• Molecular Weight: 85,745 Da
• NCBI Official Full Name: hepatocyte growth factor receptor isoform b preproprotein
• NCBI Official Synonym Full Names: MET proto-oncogene, receptor tyrosine kinase
• NCBI Official Symbol: MET
• NCBI Official Synonym Symbols: HGFR; AUTS9; RCCP2; c-Met; DFNB97
• NCBI Protein Information: hepatocyte growth factor receptor
• UniProt Protein Name: Hepatocyte growth factor receptor
• UniProt Gene Name: MET
• UniProt Synonym Gene Names: HGF receptor; SF receptor

NCBI Description

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]

Uniprot Description

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis ().