Rabbit anti-Human, Mouse Glycogen Synthase 1 Monoclonal Antibody | anti-GYS1 antibody

Glycogen Synthase 1 (Phospho-S641) Antibody

Cat. Num. AAA9431002

• Gene Names: GYS1; GSY; GYS
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: Glycogen Synthase 1; Monoclonal Antibody; Glycogen Synthase 1 (Phospho-S641) Antibody; Glycogen [starch] synthase antibody; Glycogen synthase 1 (muscle) antibody; Glycogen synthase 1 antibody; GSY antibody; GYS antibody; Gys1 antibody; GYS1_HUMAN antibody; muscle antibody; anti-GYS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: SR46-06
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-GYS1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000-1:2000; IHC: 1:50-1:200; ICC: 1:50-1:200
• Antibody Type: Recombinant Antibody
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser641 of human Glycogen synthase 1.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Phospho-Glycogen synthase 1 (S641) on different lysates using anti-Phospho-Glycogen synthase 1 (S641) antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse liver lysate, untreated Lane 3: Mouse liver lysate, treated with AP)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Glycogen synthase 1 (S641) antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Phospho-Glycogen synthase 1 (S641) antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Phospho-Glycogen synthase 1 (S641) antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Phospho-Glycogen synthase 1 (S641) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Phospho-Glycogen synthase 1 (S641) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-GYS1 antibody

Glycogen [starch] synthase belongs to the mammalian/fungal glycogen synthase family of proteins. Two forms of this protein exist, a liver form and a muscle form, both of which have the same function in the glycogen biosynthesis pathway. Glycogen synthase transfers the glycosyl residue from UDP-Glucose to the nonreducing end of -1, 4-glucan. The liver glycogen synthase protein is truncated by 34 amino acids compared to the muscle form. However, these enzymes differ significantly in their amino- and carboxyl-terminal regions. Muscle glycogen synthase serves to fuel muscular activity only and is regulated by muscle contraction and by catecholamines. Liver glycogen synthase mediates blood glucose homeostasis in response to nutritional cues. Defects in the gene encoding liver glycogen synthase results in glycogen storage disease type 0 (GSD0), a rare form of fasting ketotic hypoglycemia.

Product Categories/Family for anti-GYS1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 239049591
• NCBI GeneID: 2997
• NCBI Accession #: NP_001155059.1
• NCBI GenBank Nucleotide #: NM_001161587.1
• UniProt Accession #: P13807; Q9BTT9
• Molecular Weight: 83,786 Da
• NCBI Official Full Name: glycogen
• NCBI Official Synonym Full Names: glycogen synthase 1 (muscle)
• NCBI Official Symbol: GYS1
• NCBI Official Synonym Symbols: GSY; GYS
• NCBI Protein Information: glycogen [starch] synthase, muscle; glycogen [starch] synthase, muscle
• UniProt Protein Name: Glycogen [starch] synthase, muscle
• Protein Family: Glycogen synthase
• UniProt Gene Name: GYS1
• UniProt Synonym Gene Names: GYS
• UniProt Entry Name: GYS1_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

GYS1: muscle glycogen synthase 1. Transfers glucosyl residue from UDP-glucose to glycogen. Regulated allosterically by glucose-6-phosphate, and by PKA-mediated phosphorylation.

Protein type: EC 2.4.1.11; Transferase; Carbohydrate Metabolism - starch and sucrose

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: membrane; cytoplasm; inclusion body; cytosol

Molecular Function: protein binding; glycogen (starch) synthase activity; protein kinase binding; glucose binding

Biological Process: glycogen biosynthetic process; heart development; carbohydrate metabolic process; glucose metabolic process; pathogenesis

Disease: Glycogen Storage Disease 0, Muscle