Rabbit RhoA Polyclonal Antibody | anti-RhoA antibody

RhoA antibody

Cat. Num. AAA9404261

• Gene Names: RHOA; ARHA; ARH12; RHO12; RHOH12
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: RhoA; Polyclonal Antibody; RhoA antibody; RHOA; ARH12; ARHA; RHO12; RHOH12; anti-RhoA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total RhoA antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 193

• Applicable Applications for anti-RhoA antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500 - 1:2000
• Immunogen Type: Peptide
• Immunogen Description: Center-peptide of human RhoA.
• Target Name: RhoA
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using RHOA antibody.)

Related Product Information for anti-RhoA antibody

Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3).

Product Categories/Family for anti-RhoA antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 10835049
• NCBI GeneID: 387
• NCBI Accession #: NP_001655.1
• NCBI GenBank Nucleotide #: NM_001664.3
• UniProt Accession #: P61586; P06749; Q53HM4; Q5U024; Q9UDJ0; Q9UEJ4
• Molecular Weight: 21,768 Da
• NCBI Official Full Name: transforming protein RhoA isoform 1
• NCBI Official Synonym Full Names: ras homolog family member A
• NCBI Official Symbol: RHOA
• NCBI Official Synonym Symbols: ARHA; ARH12; RHO12; RHOH12
• NCBI Protein Information: transforming protein RhoA
• UniProt Protein Name: Transforming protein RhoA
• Protein Family: GTP-binding protein
• UniProt Gene Name: RHOA
• UniProt Synonym Gene Names: ARH12; ARHA; RHO12; h12
• UniProt Entry Name: RHOA_HUMAN

NCBI Description

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Uniprot Description

RHOA: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: G protein, monomeric, Rho; G protein, monomeric; Motility/polarity/chemotaxis; G protein; Oncoprotein

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: apical junction complex; focal adhesion; cytoskeleton; lamellipodium; plasma membrane; midbody; cell cortex; cytosol; cell junction; cleavage furrow

Molecular Function: GTPase activity; protein binding; myosin binding; GTP binding

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; negative chemotaxis; positive regulation of NF-kappaB import into nucleus; regulation of axonogenesis; regulation of cell migration; Rho protein signal transduction; regulation of osteoblast proliferation; substantia nigra development; small GTPase mediated signal transduction; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of axonogenesis; positive regulation of cytokinesis; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apical junction assembly; phosphoinositide-mediated signaling; regulation of small GTPase mediated signal transduction; positive regulation of axonogenesis; actin cytoskeleton organization and biogenesis; blood coagulation; positive regulation of neuron differentiation; vascular endothelial growth factor receptor signaling pathway