Rabbit GYS1 Polyclonal Antibody | anti-GYS1 antibody

GYS1 Polyclonal Antibody

Cat. Num. AAA3012794

• Gene Names: GYS1; GSY; GYS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: GYS1; Polyclonal Antibody; GYS1 Polyclonal Antibody; Glycogen [starch] synthase; Glycogen synthase 1 (muscle); Glycogen synthase 1; GSY; GYS; Gys1; muscle; anti-GYS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GYS1 polyclonal antibody detects endogenous levels of GYS1 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 673

• Applicable Applications for anti-GYS1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Glycogen synthase on Hela lysates using anti- Glycogen synthase antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining Glycogen synthase in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-GYS1 antibody

Glycogen [starch] synthase belongs to the mammalian/fungal glycogen synthase family of proteins. Two forms of this protein exist, a liver form and a muscle form, both of which have the same function in the glycogen biosynthesis pathway. Glycogen synthase transfers the glycosyl residue from UDP-Glucose to the nonreducing end of alpha-1, 4-glucan. The liver glycogen synthase protein is truncated by 34 amino acids compared to the muscle form. However, these enzymes differ significantly in their amino- and carboxyl-terminal regions. Muscle glycogen synthase serves to fuel muscular activity only and is regulated by muscle contraction and by catecholamines. Liver glycogen synthase mediates blood glucose homeostasis in response to nutritional cues. Defects in the gene encoding liver glycogen synthase results in glycogen storage disease type 0 (GSD0), a rare form of fasting ketotic hypoglycemia.

NCBI and Uniprot Product Information

• NCBI GI #: 239049591
• NCBI GeneID: 2997
• NCBI Accession #: NP_001155059.1
• NCBI GenBank Nucleotide #: NP_001155059.1
• UniProt Accession #: P13807; Q9Z1E4; A2RRU1
• Molecular Weight: 84kDa
• NCBI Official Full Name: glycogen
• NCBI Official Synonym Full Names: glycogen synthase 1
• NCBI Official Symbol: GYS1
• NCBI Official Synonym Symbols: GSY; GYS
• NCBI Protein Information: glycogen [starch] synthase, muscle
• UniProt Protein Name: Glycogen [starch] synthase, muscle
• Protein Family: Glycogen [starch] synthase
• UniProt Gene Name: GYS1
• UniProt Synonym Gene Names: GYS
• UniProt Entry Name: GYS1_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

GYS1: muscle glycogen synthase 1. Transfers glucosyl residue from UDP-glucose to glycogen. Regulated allosterically by glucose-6-phosphate, and by PKA-mediated phosphorylation.

Protein type: EC 2.4.1.11; Transferase; Carbohydrate Metabolism - starch and sucrose

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: membrane; cytoplasm; inclusion body; cytosol

Molecular Function: protein binding; glycogen (starch) synthase activity; protein kinase binding; glucose binding

Biological Process: glycogen biosynthetic process; heart development; carbohydrate metabolic process; glucose metabolic process; pathogenesis

Disease: Glycogen Storage Disease 0, Muscle