infectious bursal disease virus Polyprotein Recombinant Protein | PP recombinant protein

Recombinant Avian infectious bursal disease virus Polyprotein

Cat. Num. AAA1265131

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: infectious bursal disease virus Polyprotein; Recombinant Avian infectious bursal disease virus Polyprotein; PP recombinant protein

• Host: E Coli
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid containing glycerol
• Sequence Positions: 723-1012aa; Partial
• Sequence: RFPHNPRDWDRLPYLNLPYLPPNAGRQYHLAMAASEFKETPELESAVRAMEAAANVDPLFQSALSVFMWLEENGIVTDMANFALSDPNAHRMRNFLANAPQAGSKSQRAKYGTAGYGVEARGPTPEEAQREKDTRISKKMETMGIYFATPEWVALNGHRGPSPGQLKYWQNTREIPDPNEDYLDYVHAEKSRLASEEQILRAATSIYGAPGQAEPPQAFIDEVAKVYEINHGRGPNQEQMKDLLLTAMEMKHRNPRRALPKPKPKPNAPTQRPPGRLGRWIRTVSDEDLE
• Sequence Length: 1012

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for PP recombinant protein

Capsid protein VP2 self assbles to form an icosahedral capsid with a T=13 symmetry, about 70 nm in diameter, and consisting of 260 VP2 trimers. The capsid encapsulates the genomic dsRNA. VP2 is also involved in attachment and entry into the host cell by interacting with host ITGA4/ITGB1. The precursor of VP2 plays an important role in capsid assembly. First, pre-VP2 and VP2 oligomers assble to form a procapsid. Then, the pre-VP2 intermediates may be processed into VP2 proteins by proteolytic cleavage mediated by VP4 to obtain the mature virion. The final capsid is composed of pentamers and hexamers but VP2 has a natural tendency to assble into all-pentameric structures. Therefore pre-VP2 may be required to allow formation of the hexameric structures. Protease VP4 is a serine protease that cleaves the polyprotein into its final products. Pre-VP2 is first partially cleaved, and may be completely processed by VP4 upon capsid maturation.

References

Nucleotide sequence of infectious bursal disease virus genome segment A delineates two major open reading frames.Spies U., Mueller H., Becht H.Nucleic Acids Res. 17:7982-7982(1989) A comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region in VP2.Bayliss C.D., Spies U., Shaw K., Peters R.W., Papageorgiou A., Mueller H., Boursnell M.E.G.J. Gen. Virol. 71:1303-1312(1990) Role of Ser-652 and Lys-692 in the protease activity of infectious bursal disease virus VP4 and identification of its substrate cleavage sites.Lejal N., Da Costa B., Huet J.-C., Delmas B.J. Gen. Virol. 81:983-992(2000) The capsid of infectious bursal disease virus contains several small peptides arising from the maturation process of pVP2.Da Costa B., Chevalier C., Henry C., Huet J.-C., Petit S., Lepault J., Boot H., Delmas B.J. Virol. 76:2393-2402(2002) Structure of birnavirus-like particles determined by combined electron cryomicroscopy and X-ray crystallography.Pous J., Chevalier C., Ouldali M., Navaza J., Delmas B., Lepault J.J. Gen. Virol. 86:2339-2346(2005) The birnavirus crystal structure reveals structural relationships among icosahedral viruses.Coulibaly F., Chevalier C., Gutsche I., Pous J., Navaza J., Bressanelli S., Delmas B., Rey F.A.Cell 120:761-772(2005)

NCBI and Uniprot Product Information

• NCBI GI #: 130563
• UniProt Accession #: P15480
• Molecular Weight: 36.8 kDa
• NCBI Official Full Name: Structural polyprotein
• UniProt Protein Name: Structural polyprotein
• UniProt Gene Name: PP
• UniProt Synonym Gene Names: Pre-VP2; p1; p2; p3; p4; NS
• UniProt Entry Name: POLS_IBDVC

Uniprot Description

Capsid protein VP2 self assembles to form an icosahedral capsid with a T=13 symmetry, about 70 nm in diameter, and consisting of 260 VP2 trimers. The capsid encapsulates the genomic dsRNA. VP2 is also involved in attachment and entry into the host cell by interacting with host ITGA4/ITGB1 ().