Mouse KDEL Monoclonal Antibody | anti-KDEL antibody

KDEL monoclonal antibody (10C3)

Cat. Num. AAA565113

• Gene Names: Hspa5; BIP; GRP78
• Reactivity: Avian, Insect, Mammalian, Plant, Yeast
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Protein G affinity purified.
• Synonyms: KDEL; Monoclonal Antibody; KDEL monoclonal antibody (10C3); Lys-Asp-Glu-Leu; Lysine-aspartic acid-glutamate-leucine; anti-KDEL antibody

• Host: Mouse
• Reactivity: Avian, Insect, Mammalian, Plant, Yeast
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 10C3
• Specificity: Recognizes proteins containing the KDEL sequence.
• Purity/Purification: Protein G affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 654

• Applicable Applications for anti-KDEL antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemsitry (IHC)-Paraffin, Immunoprecipitation (IP), Western Blot (WB), Electron Microscopy (EM)
• Application Notes: Western Blot (1:1,000, colorimetric). Detects three bands of ~94kDa, ~78 kDa and ~55kDa by Western blot.; Optimal conditions muse be determined by individually for each application.
• Immunogen: Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.
• Source: Purified from ascites.
• Preparation and Storage: Shipping: shipped on blue ice.; Storage:; Shor-term: 4 degree C; Long term: -20 degree C.

Western Blot (WB)

(Western blot analysis: Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked).)

Immunofluorescence (IF)

(Immunofluorescence analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12 using KDEL (Grp78, Grp94) mAb (10C3).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10µg/ml.)

Related Product Information for anti-KDEL antibody

The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.

Product Categories/Family for anti-KDEL antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 121574
• NCBI GeneID: 25617
• UniProt Accession #: P06761
• Molecular Weight: 72,347 Da
• NCBI Official Full Name: 78 kDa glucose-regulated protein
• NCBI Official Synonym Full Names: heat shock protein family A member 5
• NCBI Official Symbol: Hspa5
• NCBI Official Synonym Symbols: BIP; GRP78
• NCBI Protein Information: 78 kDa glucose-regulated protein
• UniProt Protein Name: 78 kDa glucose-regulated protein
• Protein Family: KDEL motif-containing protein
• UniProt Gene Name: Hspa5
• UniProt Synonym Gene Names: Grp78; GRP-78; BiP
• UniProt Entry Name: GRP78_RAT

NCBI Description

secreted protein of the endoplasmic reticulum; may be involved in the assembly of secreted and membrane-bound proteins [RGD, Feb 2006]

Uniprot Description

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Heat shock protein; Chaperone

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum membrane; ER-Golgi intermediate compartment; focal adhesion; integral to endoplasmic reticulum membrane; melanosome; membrane; midbody; mitochondrion; myelin sheath; nucleus; plasma membrane; signalosome; smooth endoplasmic reticulum

Molecular Function: ATP binding; ATPase activity; enzyme binding; glycoprotein binding; misfolded protein binding; protein binding; protein domain specific binding; ribosome binding; ubiquitin protein ligase binding; unfolded protein binding

Biological Process: cellular response to glucose starvation; cerebellar Purkinje cell layer development; cerebellum structural organization; ER overload response; negative regulation of apoptosis; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration; positive regulation of protein ubiquitination; proteolysis involved in cellular protein catabolic process; substantia nigra development; unfolded protein response, activation of signaling protein activity