Rat ORC6 Monoclonal Antibody | anti-ORC6 antibody

ORC6 (Origin Recognition Complex Subunit 6)

Cat. Num. AAA603137

• Gene Names: ORC6; ORC6L
• Reactivity: Hamster, Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Ascites; Ascites.
• Synonyms: ORC6; Monoclonal Antibody; ORC6 (Origin Recognition Complex Subunit 6); Anti -ORC6 (Origin Recognition Complex Subunit 6); anti-ORC6 antibody

• Host: Rat
• Reactivity: Hamster, Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 7H47
• Specificity: Recognizes endogenous levels ot total ORC6 protein. Does not crossreact with other ORC subunits.
• Purity/Purification: Ascites; Ascites.
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.

• Applicable Applications for anti-ORC6 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: Western blot 1:1000; Immunoprecipitation 1:50
• Immunogen: Full length recombinant human ORC6
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from various cell lines, using MBS603137.)

Western Blot (WB)

(Immunoprecipitation of ORC6 from Jurkat cell lysates followed by Western blot, using MBS603137.)

Related Product Information for anti-ORC6 antibody

The origin recognition complex (ORC) is a highly conserved heterohexameric protein complex that associates with DNA at or near sites of initiation of DNA replication. All six ORC subunits are essential for initiation of DNA replication (1-3), and ORC may be involved in regulation of gene expression in response to stress (4). ORC binding to DNA permits the ordered binding of other proteins such as cdc6 and MCMs to form pre-replication complexes (Pre-RCs). Pre-RCs form between telophase and early G1 phase of the cell cycle and are inactivated at the onset of DNA synthesis, allowing coordinated regulation of DNA replication and cell division (5). Modification of one or more of the six ORC subunits may be responsible for its inactivation during S phase, but the chromatin binding behavior of the ORC subunits during the cell division cycle is still under investigation (6-7).

Product Categories/Family for anti-ORC6 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 7657427
• NCBI GeneID: 23594
• NCBI Accession #: NP_055136.1
• NCBI GenBank Nucleotide #: NM_014321.3
• UniProt Accession #: Q9Y5N6; B3KN89; Q9H806
• Molecular Weight: 28,107 Da
• NCBI Official Full Name: origin recognition complex subunit 6
• NCBI Official Synonym Full Names: origin recognition complex, subunit 6
• NCBI Official Symbol: ORC6
• NCBI Official Synonym Symbols: ORC6L
• NCBI Protein Information: origin recognition complex subunit 6; OTTHUMP00000163361
• UniProt Protein Name: Origin recognition complex subunit 6
• Protein Family: Origin recognition complex
• UniProt Gene Name: ORC6
• UniProt Synonym Gene Names: ORC6L
• UniProt Entry Name: ORC6_HUMAN

NCBI Description

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis. [provided by RefSeq]

Uniprot Description

ORC6L: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. ORC is composed of six subunits. In human, ORC is cell cycle-dependent regulated: it is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with DBF4. Belongs to the ORC6 family.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 16q12

Cellular Component: nucleoplasm; membrane; plasma membrane; nucleus; nuclear origin of replication recognition complex; origin recognition complex

Molecular Function: protein binding; DNA binding

Biological Process: mitotic cell cycle; DNA-dependent DNA replication; DNA replication; G1/S transition of mitotic cell cycle

Disease: Meier-gorlin Syndrome 3