Rabbit anti-Rat GLS2 Polyclonal Antibody | anti-GLS2 antibody

GLS2 Antibody

Cat. Num. AAA150300

• Gene Names: GLS2; GA; GLS; LGA; hLGA
• Reactivity: Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: GLS2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: GLS2; Polyclonal Antibody; GLS2 Antibody; GA; GLS; LGA; hLGA; Glutaminase liver isoform; mitochondrial; L-glutaminase; glutaminase 2 (liver; mitochondrial); anti-GLS2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Multiple isoforms of GLS2 are known to exist.
• Purity/Purification: GLS2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 602

• Applicable Applications for anti-GLS2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.; ; Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.; ; Optimal dilutions for each application to be determined by the researcher.
• Conjugate: Unconjugated
• Immunogen: GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2.
• Buffer: GLS2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: GLS2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of GLS2 in rat liver tissue lysate with GLS2 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of GLS2 in rat liver tissue with GLS2 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of GLS2 in rat liver cells with GLS2 antibody at 20 5 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of GLS2 in mouse brain tissue with GLS2 Antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of GLS2 in mouse brain tissue with GLS2 Antibody at 20 μg/mL.Green: GLS2 antibody (6217)Red: Phylloidin stainingBlue: DAPI staining)

Related Product Information for anti-GLS2 antibody

GLS2 Antibody: Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.

NCBI and Uniprot Product Information

• NCBI GI #: 20336214
• NCBI GeneID: 27165
• NCBI Accession #: NP_037399
• NCBI GenBank Nucleotide #: NM_013267.3
• UniProt Accession #: Q9UI32; Q8IX91; Q9NYY2; Q9UI31; B7Z8Q9
• Molecular Weight: Predicted: 36; 55; 62; 66 kDa; ; Observed: 55; 62 kDa
• NCBI Official Full Name: glutaminase liver isoform, mitochondrial isoform 1
• NCBI Official Synonym Full Names: glutaminase 2 (liver, mitochondrial)
• NCBI Official Symbol: GLS2
• NCBI Official Synonym Symbols: GA; GLS; LGA; hLGA
• NCBI Protein Information: glutaminase liver isoform, mitochondrial; glutaminase I; breast cell glutaminase; L-glutamine amidohydrolase; phosphate-activated glutaminase; phosphate-dependent glutaminase
• UniProt Protein Name: Glutaminase liver isoform, mitochondrial
• Protein Family: Glucosidase
• UniProt Gene Name: GLS2
• UniProt Synonym Gene Names: GA; GLS
• UniProt Entry Name: GLSL_HUMAN

NCBI Description

The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

GLS2: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. Belongs to the glutaminase family.

Protein type: Mitochondrial; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; EC 3.5.1.2; Hydrolase; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: protein binding; glutaminase activity

Biological Process: amino acid metabolic process; regulation of apoptosis; synaptic transmission; glutamate secretion; neurotransmitter secretion; glutamine metabolic process; amino acid biosynthetic process