Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.brain section using GLS2 Antibody (C-term E513). MBS9205614 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Rabbit GLS2 Polyclonal Antibody | anti-GLS2 antibody
GLS2 Antibody (C-term E513)
WB~~1:1000
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.brain section using GLS2 Antibody (C-term E513). MBS9205614 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.brain section using GLS2 Antibody (C-term E513). MBS9205614 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Western Blot (WB)
(Western blot analysis of lysate from human brain tissue lysate, using GLS2 Antibody (C-term E513). MBS9205614 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(Western blot analysis of lysate from human brain tissue lysate, using GLS2 Antibody (C-term E513). MBS9205614 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(GLS2 Antibody (C-term E513) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GLS2 antibody detected the GLS2 protein (arrow).)
Western Blot (WB)
(GLS2 Antibody (C-term E513) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GLS2 antibody detected the GLS2 protein (arrow).)
Immunohistochemistry (IHC)
(GLS2 antibody(C-term E513) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLS2 antibody(C-term E513) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GLS2 antibody(C-term E513) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLS2 antibody(C-term E513) for immunohistochemistry. Clinical relevance has not been evaluated.)
phosphate-activated glutaminase that catalyzes the hydrolysis of
glutamine to stoichiometric amounts of glutamate and ammonia. This
protein is functionally similar to the kidney glutaminase but is a
little smaller in size. Originally thought to be liver-specific,
this protein has been found in other tissues as well. At least one
transcribed pseudogene has been found for this gene. [provided by
RefSeq].
Suzuki, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(16):7461-7466(2010)
Szeliga, M., et al. Glia 57(9):1014-1023(2009)
Tian, C., et al. J. Neurochem. 105(3):994-1005(2008)
Maeshima, H., et al. Prog. Neuropsychopharmacol. Biol. Psychiatry 31(7):1410-1418(2007)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]
Uniprot Description
GLS2: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. Belongs to the glutaminase family.
Protein type: Amino Acid Metabolism - arginine and proline; Hydrolase; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 3.5.1.2; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Energy Metabolism - nitrogen; Mitochondrial
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: mitochondrion; mitochondrial matrix
Molecular Function: protein binding; glutaminase activity
Biological Process: regulation of apoptosis; amino acid metabolic process; synaptic transmission; glutamate secretion; neurotransmitter secretion; glutamine metabolic process; amino acid biosynthetic process
Research Articles on GLS2
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Product Notes
The GLS2 gls2 (Catalog #AAA9205614) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 498-524. The GLS2 Antibody (C-term E513) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's GLS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). IHC~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the GLS2 gls2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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