Typical Testing Data/Standard Curve
Mouse IL-17E ELISA Kit | IL25 elisa kit
Mouse IL-17E ELISA Kit
Reactivity
Mouse
Applications
ELISA
Synonyms
IL-17E; Mouse IL-17E ELISA Kit; IL17E; Interleukin-25; IL-25; Interleukin-17E; IL25 elisa kit
Reactivity
Mouse
Specificity
The Mouse IL-17E ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IL-17E proteins within the range of 31.3 pg/ml - 2000 pg/ml.
Sequence Length
169
Applicable Applications for IL25 elisa kit
Sandwich ELISA (EIA)
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
15 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for IL25 elisa kit
Principle of the Assay||The Mouse IL-17E ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-17E in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse IL-17E coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17E present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-17E antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17E bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-kappaB activation, and stimulate the production of IL8. What's more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.
Background/Introduction: Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-kappaB activation, and stimulate the production of IL8. What's more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
19,210 Da
NCBI Official Full Name
IL25 (Interleukin 25)
UniProt Protein Name
IL25
Protein Family
UniProt Gene Name
Il25
UniProt Entry Name
Q8VHH8_MOUSE
Uniprot Description
IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide; Cytokine
Cellular Component: extracellular space
Molecular Function: cytokine activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-17E receptor binding
Biological Process: cell surface receptor linked signal transduction; eosinophil differentiation; inflammatory response; inflammatory response to antigenic stimulus; interleukin-13 production; interleukin-5 production; positive regulation of transcription from RNA polymerase II promoter; response to fungus; response to nematode
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Product Notes
The Mouse IL25 il25 (Catalog #AAA8244556) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244556 ELISA Kit recognizes Mouse IL25. AAA Biotech's IL-17E can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the IL25 il25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-17E, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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