Rabbit anti-Human, Mouse SPAG4 Polyclonal Antibody | anti-SPAG4 antibody

SPAG4 Rabbit pAb

Cat. Num. AAA4758338

• Gene Names: SPAG4; SUN4; CT127
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: SPAG4; Polyclonal Antibody; SPAG4 Rabbit pAb; SUN4; CT127; anti-SPAG4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-SPAG4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000
• Immunogen: Synthetic peptide of human SPAG4
• Conjugation: Unconjugated
• Molecular Function: The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development.
• Modification: Unmodified
• Cell Position: Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Membrane, Nucleus
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: NIH/3T3 cell lysate, Primary antibody: 860986(SPAG4 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 860986(SPAG4 Antibody) at dilution 1/70. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 860986(SPAG4 Antibody) at dilution 1/70. (Original magnification: ×200))

Product Categories/Family for anti-SPAG4 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 11037055
• NCBI GeneID: 6676
• NCBI Accession #: NP_003107
• NCBI GenBank Nucleotide #: NM_003116.1
• UniProt Accession #: Q9NPE6; O43648
• Molecular Weight: 48,165 Da
• NCBI Official Full Name: sperm-associated antigen 4 protein
• NCBI Official Synonym Full Names: sperm associated antigen 4
• NCBI Official Symbol: SPAG4
• NCBI Official Synonym Symbols: SUN4; CT127
• NCBI Protein Information: sperm-associated antigen 4 protein
• UniProt Protein Name: Sperm-associated antigen 4 protein
• Protein Family: Sperm-associated antigen 4 protein
• UniProt Gene Name: SPAG4
• UniProt Synonym Gene Names: SUN4
• UniProt Entry Name: SPAG4_HUMAN

NCBI Description

The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. [provided by RefSeq, Jul 2008]