Mouse anti-Human Galectin-8 Monoclonal Antibody | anti-LGALS8 antibody

Galectin-8, Human, mAb 106.1

Cat. Num. AAA584375

• Gene Names: LGALS8; Gal-8; PCTA1; PCTA-1; Po66-CBP
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: Galectin-8; Monoclonal Antibody; Human; mAb 106.1; Gal-8; PCTA1; PCTA-1; Po66-CBP; LGALS8; anti-LGALS8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 359

• Applicable Applications for anti-LGALS8 antibody: Flow Cytometry (FC/FACS), FS, Immunoassay (IA)
• Application Notes: Application Use: For Flow cytometry and Functional Studies dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.-
• Immunogen: recombinant rat Galectin-8
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label-

Related Product Information for anti-LGALS8 antibody

Product Description: Galectins are animal lectins that can specifically bind beta-galactosides. Thirteen galectins have already been described, belonging to three different groups: prototype, tandem-repeat and chimeric. Galectins are involved in cellular interactions and neoplastic transformations. Galectin-8, is a 34 kDa protein made of tandem-repeat carbohydrate recognition domains (CRDs), joined by a "link" peptide. Structurally, galectin-8 belongs to the tandem-repeat type galectins. The galectin-8 gene (LGALS8) encodes numerous mRNAs by alternate splicing and the presence of three unusual polyadenylation signals. These mRNAs could encode for six isoforms of galectin-8, of which three belong to the tandem-repeat galectin group (with two carbohydrate binding domains) and the three others to the prototype group (one carbohydrate binding domain). Galectin-8, like other galectins, is a secreted protein. Upon secretion galectin-8 acts as a physiological modulator of cell adhesion. When immobilized, it functions as a matrix protein equipotent to fibronectin in promoting cell adhesion by ligation and clustering of a selective subset of cell surface integrin receptors. Although galectin-8 is a fairly abundant protein, it is not ubiquitously expressed. It shows a micro-clustering pattern reminiscent of that seen with proteins associated with mitochondria, the Golgi or trans-Golgi membranes. Studies of galectin-8 revealed that galectin-8 positively or negatively regulates cell adhesion, depending on the extracellular context. Cell adhesion to galectin-8 triggers integrin-mediated signalling cascades such as Tyr phosphorylation of FAK and paxillin. Galectin-8 is widely expressed in normal tissues (brain, breast, colon, retina, kidney, pancreas, placenta, spleen, testis, uterus, vascular, esophagus and heart) as well as in tumor tissues (brain, breast, colon, germ cells, head and neck, kidney, muscles, ovary, pancreas, thyroid, placenta, prostate, uterus, lung, stomach and esophagus).

References

1. Levy, Y et al; Galectin-8 functions as a matricellular modulator of cell adhesion. J Biol Chem 2001, 276: 31285. 2. Eshkar Sebban, L et al; The involvement of CD44 and its novel ligand Galectin-8 in apoptotic regulation of autoimmune inflammation. J Immunol 2007, 179: 1225.

NCBI and Uniprot Product Information

• NCBI GI #: 42544193
• NCBI GeneID: 3964
• NCBI Accession #: NP_963839.1
• NCBI GenBank Nucleotide #: NM_201545.2
• Molecular Weight: 40,397 Da
• NCBI Official Full Name: galectin-8 isoform a
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 8
• NCBI Official Symbol: LGALS8
• NCBI Official Synonym Symbols: Gal-8; PCTA1; PCTA-1; Po66-CBP
• NCBI Protein Information: galectin-8; galectin 8; galectin-8g; Po66 carbohydrate binding protein; po66 carbohydrate-binding protein; prostate carcinoma tumor antigen 1
• UniProt Protein Name: Galectin-8
• Protein Family: Galectin
• UniProt Gene Name: LGALS8
• UniProt Synonym Gene Names: Gal-8; Po66-CBP; PCTA-1
• UniProt Entry Name: LEG8_HUMAN

NCBI Description

This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

LGALS8: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: extracellular space; membrane; cytoplasm

Molecular Function: protein binding; carbohydrate binding

Biological Process: plasma cell differentiation; T cell costimulation