Human Neurogenic locus notch homolog protein 2, NOTCH2 ELISA Kit | NOTCH2 elisa kit

Human Neurogenic locus notch homolog protein 2, NOTCH2 ELISA Kit

Cat. Num. AAA166618

• Reactivity: Human
• Synonyms: Neurogenic locus notch homolog protein 2; NOTCH2; Human Neurogenic locus notch homolog protein 2; NOTCH2 ELISA Kit; NOTCH2 elisa kit

• Reactivity: Human
• Sequence Length: 2471

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.5ng/ml - 100ng/ml
• Sensitivity: 0.26ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOTCH2 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Neurogenic Locus Notch Homolog Protein 2 (also known as NOTCH2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human NOTCH2 antibody. NOTCH2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human NOTCH2 Antibody is added and binds to NOTCH2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NOTCH2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human NOTCH2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 767172165
• NCBI GeneID: 29492
• NCBI Accession #: NP_077334.2
• NCBI GenBank Nucleotide #: NM_024358.2
• UniProt Accession #: Q9QW30
• Molecular Weight: 265,370 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 2
• NCBI Official Synonym Full Names: notch 2
• NCBI Official Symbol: Notch2
• NCBI Protein Information: neurogenic locus notch homolog protein 2
• UniProt Protein Name: Neurogenic locus notch homolog protein 2
• Protein Family: Neurogenic locus notch homolog protein
• UniProt Gene Name: Notch2
• UniProt Synonym Gene Names: Notch 2

NCBI Description

transmembrane protein that may be involved in cellular interactions that specify cell fate [RGD, Feb 2006]

Uniprot Description

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells ().