Rabbit anti-Human Galectin-1/LGALS1  Monoclonal Antibody | anti-LGALS1 antibody

Rabbit anti Human Galectin-1/LGALS1  Monoclonal Antibody

Cat. Num. AAA2543913

• Gene Names: LGALS1; GBP; GAL1
• Reactivity: Human
• Applications: ELISA
• Synonyms: Galectin-1/LGALS1 ; Monoclonal Antibody; Rabbit anti Human Galectin-1/LGALS1 Monoclonal Antibody; anti-LGALS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 135

• Applicable Applications for anti-LGALS1 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human Galectin1. The detection limit for Human Galectin1 is approximately 0.0012 ng/well.
• Immunogen: Recombinant Human Galectin-1 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-LGALS1 antibody

Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state in order to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumour growth, has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.

NCBI and Uniprot Product Information

• NCBI GI #: 4504981
• NCBI GeneID: 3956
• NCBI GenBank Nucleotide #: NM_002305.3
• Molecular Weight: 14,716 Da
• NCBI Official Full Name: galectin-1
• NCBI Official Synonym Full Names: galectin 1
• NCBI Official Symbol: LGALS1
• NCBI Official Synonym Symbols: GBP; GAL1
• NCBI Protein Information: galectin-1
• UniProt Protein Name: Galectin-1
• UniProt Gene Name: LGALS1
• UniProt Synonym Gene Names: Gal-1; HLBP14
• UniProt Entry Name: LEG1_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Protein type: Cell adhesion; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; extracellular matrix; extracellular space; intracellular

Molecular Function: protein binding; signal transducer activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of virion penetration into host cell; regulation of apoptosis