Rabbit UBF Polyclonal Antibody | anti-UBF antibody

UBF (Phospho-Ser484) Antibody

Cat. Num. AAA9503413

• Gene Names: UBTF; UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: UBF; Polyclonal Antibody; UBF (Phospho-Ser484) Antibody; Autoantigen NOR-90; Nucleolar transcription factor 1; TCFUBF; UBF-1; UBF1; UBTF; Upstream binding factor 1; anti-UBF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: UBF (Phospho-Ser484) Antibody detects endogenous levels of UBF only when phosphorylated at Ser484.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 727

• Applicable Applications for anti-UBF antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:1000
• Target Modification: Phospho
• Modification Sites: H:S484; M:S484; R:S484
• Immunogen: The antiserum was produced against synthesized peptide derived from human UBF around the phosphorylation site of Ser484.
• Immunogen Range: 451-500
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using UBF (Phospho-Ser484) Antibody. The picture on the right is blocked with the phospho peptide.)

Western Blot (WB)

(Western blot analysis of lysates from HeLa cells treated with calyculinA 50ng/ml 30', using UBF (Phospho-Ser484) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 115529451
• NCBI GeneID: 7343
• NCBI Accession #: NP_001070151.1
• NCBI GenBank Nucleotide #: NM_001076683.1
• UniProt Accession #: P17480; A8K6R8
• Molecular Weight: 89 kDa
• NCBI Official Full Name: nucleolar transcription factor 1 isoform b
• NCBI Official Synonym Full Names: upstream binding transcription factor, RNA polymerase I
• NCBI Official Symbol: UBTF
• NCBI Official Synonym Symbols: UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90
• NCBI Protein Information: nucleolar transcription factor 1
• UniProt Protein Name: Nucleolar transcription factor 1
• UniProt Gene Name: UBTF
• UniProt Synonym Gene Names: UBF; UBF1; UBF-1

NCBI Description

This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011]

Uniprot Description

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.