Mouse anti-Human SPHK1 Monoclonal Antibody | anti-SPHK1 antibody

SPHK1 (Sphingosine Kinase 1, SK 1, SPHK, SPK 1, SPK) (FITC)

Cat. Num. AAA6149860

• Gene Names: SPHK1; SPHK
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPHK1; Monoclonal Antibody; SPHK1 (Sphingosine Kinase 1; SK 1; SPHK; SPK 1; SPK) (FITC); EC=2.7.1.91; anti-SPHK1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1D6
• Specificity: Recognizes human SPHK1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-SPHK1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-385 from SPHK1 (AAH08040) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLLLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (68.35kD).)

Western Blot (WB)

(Western Blot analysis of SPHK1 expression in transfected 293T cell line by SPHK1 monoclonal antibody. Lane 1: SPHK1 transfected lysate (43.9kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged SPHK1 is ~1ng/ml as a capture antibody.)

Product Categories/Family for anti-SPHK1 antibody

Antibodies; Protein Kinases

References

1. The sphingosine kinase 1 and S1P1 axis specifically counteracts LPS-induced IL-12p70 production in immune cells of the spleen. Schroder M, Richter C, Juan MH, Maltusch K, Giegold O, Quintini G, Pfeilschifter JM, Huwiler A, Radeke HH.Mol Immunol. 2011 Mar 22. 2. The role of Eag and HERG channels in cell proliferation and apoptotic cell death in SK-OV-3 ovarian cancer cell line. Asher V, Warren A, Shaw R, Sowter H, Bali A, Khan R.Cancer Cell Int. 2011 Mar 10;11:6. 3. Effect of HFE Variants on Sphingolipid Expression by SH-SY5Y Human Neuroblastoma Cells. Ali-Rahmani F, Hengst JA, Connor JR, Schengrund CL.Neurochem Res. 2011 Jan 18. 4. Sphingosine kinases regulate NOX2 activity via p38 MAPK-dependent translocation of S100A8/A9. Schenten V, Melchior C, Steinckwich N, Tschirhart EJ, Brechard S.J Leukoc Biol. 2011 Apr;89(4):587-96. Epub 2011 Jan 13. 5. Increased radiation sensitivity of head and neck squamous cell carcinoma with sphingosine kinase 1 inhibition. Sinha UK, Schorn VJ, Hochstim C, Chinn SB, Zhu S, Masood R.Wiley InterScience. DOI: 10.1002/ hed.21418 6. A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart. Wang S, Zhang Z, Lin X, Xu DS, Feng Y, Ding K.Glycobiology. 2010 Jan;20(4):473-84. Epub 2009 Dec 15. 7. G{alpha}q-mediated plasma membrane translocation of sphingosine kinase-1 and cross-activation of S1P receptors. Ter Braak M, Danneberg K, Lichte K, Liphardt K, Ktistakis NT, Pitson SM, Hla T, Jakobs KH, Heringdorf DM.Biochim Biophys Acta. 2009 Feb 2. 8. Sphingosine kinase 1 is essential for proteinase-activated receptor-1 signalling in epithelial and endothelial cells. Billich A, Urtz N, Reuschel R, Baumruker T.Int J Biochem Cell Biol. 2009 Jul;41(7):1547-55. Epub 2009 Jan 8.

NCBI and Uniprot Product Information

• NCBI GI #: 33872079
• NCBI GeneID: 8877
• Molecular Weight: ~71 kDa
• NCBI Official Full Name: Homo sapiens sphingosine kinase 1, mRNA
• NCBI Official Synonym Full Names: sphingosine kinase 1
• NCBI Official Symbol: SPHK1
• NCBI Official Synonym Symbols: SPHK
• NCBI Protein Information: sphingosine kinase 1

NCBI Description

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]