Testing Data
(Mouse brain membrane lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS631731 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein GABA A Receptor 2,3 Chain (~55kD).)
Mouse GABA Receptor A, beta Monoclonal Antibody | anti-gab-1 antibody
GABA Receptor A, beta (Gamma-aminobutyric Acid Receptor, GABRA)
Reactivity
Bovine, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
GABA Receptor A; beta; Monoclonal Antibody; beta (Gamma-aminobutyric Acid Receptor; GABRA); Anti -GABA Receptor A; anti-gab-1 antibody
Host
Mouse
Reactivity
Bovine, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q69
Specificity
Recognizes the beta2 and beta3 chain of bovine GABA Receptor A at ~55kD. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4.
Applicable Applications for anti-gab-1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:500 detected GABA A Receptor beta 2,3 chain on 10ug of mouse brain membrane lysate. Mouse brain membrane lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with G1015-17. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunohistochemistry: 10-20ug/ml
Dilution: Western Blot: 1:500 detected GABA A Receptor beta 2,3 chain on 10ug of mouse brain membrane lysate. Mouse brain membrane lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with G1015-17. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunohistochemistry: 10-20ug/ml
Immunogen
Purified GABA/benzodiazepine receptor from bovine cortex. Epitope: Extracellular domain of the beta2 and beta3 subunits of the GABA receptor A.
Positive Control
HepG2 cell lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Mouse brain membrane lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS631731 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein GABA A Receptor 2,3 Chain (~55kD).)
Testing Data
(Mouse brain membrane lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS631731 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein GABA A Receptor 2,3 Chain (~55kD).)
Related Product Information for anti-gab-1 antibody
Gamma-aminobutyric acid tye A (GABAA) receptors are members of the neurotransmitter ligand-gated ion channels that mediate neuronal inhibition on binding GABA. The effects of GABA on GABAA receptors are modulated by a range of therapeutically important drugs, including barbiturates, anaesthetics and benzodiazepines.
Product Categories/Family for anti-gab-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
62,457 Da
NCBI Official Full Name
Gamma-aminobutyric acid receptor subunit beta
NCBI Official Symbol
gab-1
NCBI Protein Information
GABa receptor subunit family member (gab-1)
UniProt Protein Name
Gamma-aminobutyric acid receptor subunit beta
Protein Family
UniProt Gene Name
gab-1
UniProt Entry Name
GBRB_CAEEL
Uniprot Description
Function: GABA, an inhibitory neurotransmitter, mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel
By similarity. Ref.1
Subcellular location: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein
By similarity. Cell membrane; Multi-pass membrane protein
Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. [View classification]
Similar Products
Product Notes
The gab-1 gab-1 (Catalog #AAA631731) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GABA Receptor A, beta (Gamma-aminobutyric Acid Receptor, GABRA) reacts with Bovine, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GABA Receptor A, beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:500 detected GABA A Receptor beta 2,3 chain on 10ug of mouse brain membrane lysate. Mouse brain membrane lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with G1015-17. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunohistochemistry: 10-20ug/ml. Researchers should empirically determine the suitability of the gab-1 gab-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GABA Receptor A, beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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