Mouse anti-Human free PSA Monoclonal Antibody | anti-KLK3 antibody

Anti-free PSA Antibody (1D3E6)

Cat. Num. AAA847224

• Gene Names: KLK3; APS; PSA; hK3; KLK2A1
• Reactivity: Human
• Applications: ELISA
• Purity: Protein A purification
• Synonyms: free PSA; Monoclonal Antibody; Anti-free PSA Antibody (1D3E6); Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase; APS; Prostate-specific antigen; anti-KLK3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone Number: 1D3E6
• Specificity: Monoclonal antibody to detect free PSA. Free PSA monoclonal antibodies (Clone# 4E9C2, 1D3E6 and 1B4A5) recognize human free PSA protein.
• Purity/Purification: Protein A purification
• Form/Format: Colorless liquid. In PBS buffer, pH7.4, containing 0.02% sodium azide
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-KLK3 antibody: ELISA (EIA)
• Application Notes: ELISA Capture: 1-10ug/ml; ELISA Detection: 0.05-0.2ug/ml
• Immunogen: Free PSA protein purified from human seminal fluid
• Preparation and Storage: Store at -20 degree C; The antibody solution should be gently mixed before use; Shelf life: ~12 months

ELISA (EIA)

Related Product Information for anti-KLK3 antibody

Prostate-specific antigen (PSA) is produced by cells of the prostate gland. The PSA level increases in the blood of men who have prostate cancer, benign prostatic hyperplasia, or infection or inflammation of the prostate. It exists in the blood in two forms of free PSA and total PSA (PSA attached to a protein molecule). Free PSA increases more in benign prostatic hypertrophy while total PSA is associated with cancer. When Prostate cancer occurs, PSA often ranges from 2.5 to 10 ng/ml. The free PSA and total PSA are tested simultaneously to identify what kind of prostate problem a man has.Free PSA Antibody is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with free PSA protein purified from human seminal fluid

Product Categories/Family for anti-KLK3 antibody

Tumor Biomarker; Neuroscience Antibodies; Antibodies & Supporting Tools; IVD Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 71834853
• NCBI GeneID: 354
• NCBI Accession #: NP_001025218.1
• NCBI GenBank Nucleotide #: NM_001030047.1
• UniProt Accession #: P07288; Q15096; Q16272; Q86TG8; C9JXH3; G3V0H4; G3XAE3
• Molecular Weight: 28,741 Da
• NCBI Official Full Name: prostate-specific antigen isoform 3 preproprotein
• NCBI Official Synonym Full Names: kallikrein-related peptidase 3
• NCBI Official Symbol: KLK3
• NCBI Official Synonym Symbols: APS; PSA; hK3; KLK2A1
• NCBI Protein Information: prostate-specific antigen; seminin; P-30 antigen; kallikrein-3; semenogelase; gamma-seminoprotein; prostate specific antigen
• UniProt Protein Name: Prostate-specific antigen
• Protein Family: Glandular kallikrein
• UniProt Gene Name: KLK3
• UniProt Synonym Gene Names: APS; PSA; Seminin
• UniProt Entry Name: KLK3_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: Secreted; Protease; EC 3.4.21.77; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region; nucleus

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis