Rabbit anti-Human IDUA Polyclonal Antibody | anti-IDUA antibody

IDUA, ID (IDUA, Alpha-L-iduronidase) (APC)

Cat. Num. AAA6309190

• Gene Names: IDUA; IDA; MPS1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: IDUA; Polyclonal Antibody; ID (IDUA; Alpha-L-iduronidase) (APC); Alpha-L-iduronidase; anti-IDUA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IDUA.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-IDUA antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 236-264 in the central region of human IDUA.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS6000318. This demonstrates that MBS6000318 detected the IDUA protein (arrow).)

Testing Data

(All lanes: MBS6000318 (1:2000) Lane 1: human liver lysates Lane 2: human lung lysates Lysates/proteins at 20ug/lane. Secondary goat anti-rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 73kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma using MBS6000318 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Product Categories/Family for anti-IDUA antibody

Antibodies; Enzymes

References

1. Amr, K., et al. Genet Test Mol Biomarkers 13(6):761-764(2009). 2. Vazna, A., et al. Am. J. Med. Genet. A 149A (5), 965-974 (2009). 3. Sugawara, K., et al. J. Hum. Genet. 53(5):467-474(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 110611239
• NCBI GeneID: 3425
• NCBI Accession #: NP_000194.2
• NCBI GenBank Nucleotide #: NM_000203.5
• UniProt Accession #: P35475; B3KWK6
• Molecular Weight: 73,455 Da
• NCBI Official Full Name: alpha-L-iduronidase isoform a
• NCBI Official Synonym Full Names: iduronidase, alpha-L-
• NCBI Official Symbol: IDUA
• NCBI Official Synonym Symbols: IDA; MPS1
• NCBI Protein Information: alpha-L-iduronidase
• UniProt Protein Name: Alpha-L-iduronidase
• Protein Family: Alpha-L-iduronidase
• UniProt Gene Name: IDUA

NCBI Description

This gene encodes an enzyme that hydrolyzes the terminal alpha-L-iduronic acid residues of two glycosaminoglycans, dermatan sulfate and heparan sulfate. This hydrolysis is required for the lysosomal degradation of these glycosaminoglycans. Mutations in this gene that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis type I (MPS I). [provided by RefSeq, Jul 2008]