Mouse anti-Human PSA Monoclonal Antibody | anti-KLK3 antibody

Anti-PSA Antibody (4A5D10)

Cat. Num. AAA847221

• Gene Names: KLK3; APS; PSA; hK3; KLK2A1
• Reactivity: Human
• Applications: ELISA
• Purity: Protein A purification
• Synonyms: PSA; Monoclonal Antibody; Anti-PSA Antibody (4A5D10); Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase; APS; Prostate-specific antigen; anti-KLK3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone Number: 4A5D10
• Specificity: Monoclonal antibody to detect PSA. PSA monoclonal antibodies (Clone# 5B10D4, 8A9B8, 1G9G8, 4A5D10 and 1A7D4) recognize total PSA, free PSA and ACT-PSA Complex (Prostate Specific Antigen / a-1 Antichymotrypsin Complex).
• Purity/Purification: Protein A purification
• Form/Format: Colorless liquid. In PBS buffer, pH7.4, containing 0.02% sodium azide
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-KLK3 antibody: ELISA (EIA)
• Immunogen: Human total PSA purified from semenal plasma.
• Preparation and Storage: Store at -20 degree C; The antibody solution should be gently mixed before use; Shelf life: ~12 months

ELISA (EIA)

Related Product Information for anti-KLK3 antibody

Prostate-specific antigen (PSA) is also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen. It is a serine protease enzyme produced by the cells of the prostate gland. Most of PSA in the blood which is bound to serum proteins is known as total PSA, while a small amount which is not protein bound to is called free PSA. PSA liquifies the semen in the seminal coagulum and allows sperm to swim freely. PSA is often elevated in the presence of prostate cancer and in other prostate disorders. A blood test to measure PSA is considered to be the most effective test currently available for the early detection of prostate cancer. Furthermore, rising levels of PSA over time are associated with both localized and metastatic prostate cancer (CaP) .Human PSA monoclonal antibody, is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human total PSA purified from semenal plasma.

Product Categories/Family for anti-KLK3 antibody

Tumor Biomarker; Neuroscience Antibodies; Antibodies & Supporting Tools; IVD Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 71834853
• NCBI GeneID: 354
• NCBI Accession #: NP_001025218.1
• NCBI GenBank Nucleotide #: NM_001030047.1
• UniProt Accession #: P07288; Q15096; Q16272; Q86TG8; C9JXH3; G3V0H4; G3XAE3
• Molecular Weight: 28,741 Da
• NCBI Official Full Name: prostate-specific antigen isoform 3 preproprotein
• NCBI Official Synonym Full Names: kallikrein-related peptidase 3
• NCBI Official Symbol: KLK3
• NCBI Official Synonym Symbols: APS; PSA; hK3; KLK2A1
• NCBI Protein Information: prostate-specific antigen; seminin; P-30 antigen; kallikrein-3; semenogelase; gamma-seminoprotein; prostate specific antigen
• UniProt Protein Name: Prostate-specific antigen
• Protein Family: Prostate-specific antigen
• UniProt Gene Name: KLK3
• UniProt Synonym Gene Names: APS; PSA; Seminin
• UniProt Entry Name: KLK3_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: Secreted; Protease; EC 3.4.21.77; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region; nucleus

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis