Rabbit GRP1 Polyclonal Antibody | anti-GRP1 antibody

GRP1 (K400) Polyclonal Antibody

Cat. Num. AAA3003215

• Gene Names: CYTH3; GRP1; ARNO3; PSCD3; cytohesin-3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GRP1; Polyclonal Antibody; GRP1 (K400) Polyclonal Antibody; Cytohesin-3; ARF nucleotide-binding site opener 3; Protein ARNO3; General receptor of phosphoinositides 1; Grp1; PH; SEC7 and coiled-coil domain-containing protein 3; ARNO3; PSCD3; CYTH3; anti-GRP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GRP1 (K400) polyclonal antibody detects endogenous levels of GRP1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 400

• Applicable Applications for anti-GRP1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 371-420 of Human GRP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GRP1 (K400) pAb at 1:2000 dilutionLane1:3T3-L1 whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:A2780 whole cell lysate (40ug) Lane4:H1792 whole cell lysate (40ug) Lane5:A375 whole cell lysate (20ug))

Related Product Information for anti-GRP1 antibody

GRP1 (general receptor for phosphoinositides-1) contains a Pleckstrin homology (PH) domain as well as a Sec7 domain. The PH domain has high binding affinity for phosphatidylinositol 3, 4, 5-trisphosphate Ptdlns(3, 4, 5)P3), while the Sec7 homology domain is responsible for catalyzing guanine nucleotide exchange of ADP-ribosylation factor (ARF) proteins. GRP1 co-localizes with ARF6 and catalyzes GTP/GDP exchange on ARF6. It is known to interact with Ptdlns(3, 4, 5)P3 localized to the plasma membrane in vitro and may also be a Ptdlns(3, 4, 5)P3 receptor. Additionally, GRP1 may regulate protein sorting and membrane trafficking through interaction with the guanosine triphosphate ARF, and may control cell adhesion through interaction with integrins.

NCBI and Uniprot Product Information

• NCBI GI #: 13124042
• NCBI GeneID: 9265
• UniProt Accession #: O43739
• Molecular Weight: ~ 48kDa
• NCBI Official Full Name: Cytohesin-3
• NCBI Official Synonym Full Names: cytohesin 3
• NCBI Official Symbol: CYTH3
• NCBI Official Synonym Symbols: GRP1; ARNO3; PSCD3; cytohesin-3
• NCBI Protein Information: cytohesin-3
• UniProt Protein Name: Cytohesin-3
• UniProt Gene Name: CYTH3
• UniProt Synonym Gene Names: ARNO3; GRP1; PSCD3; Protein ARNO3; Grp1
• UniProt Entry Name: CYH3_HUMAN

NCBI Description

This gene encodes a member of the PSCD (pleckstrin homology, Sec7 and coiled-coil domains) family. PSCD family members have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This encoded protein is involved in the control of Golgi structure and function, and it may have a physiological role in regulating ADP-ribosylation factor protein 6 (ARF) functions, in addition to acting on ARF1. [provided by RefSeq, Jul 2008]

Uniprot Description

cytohesin 3: Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF through replacement of GDP with GTP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; GEFs; GEFs, ARF; Lipid-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 7p22.1

Cellular Component: ruffle; extrinsic to internal side of plasma membrane; plasma membrane; trans-Golgi network; cytosol

Molecular Function: phosphatidylinositol-3,4,5-triphosphate binding; ARF guanyl-nucleotide exchange factor activity

Biological Process: regulation of cell adhesion; vesicle-mediated transport; positive regulation of cell adhesion; regulation of ARF protein signal transduction; positive regulation of GTPase activity; Golgi vesicle transport