Western Blot (WB)
(I7651-58A used for the evaluation of viral nucleoprotien by immunofluorescence and western blot. The nuclear egress of vRNPs late in infection leads to PKR activation. (A) A549 cells grown on glass cover slips were infected with WT or mutant virus #4 at an MOI of 1. Cells were mock treated or complemented with LMB starting at 3 hours p.i.. At 8, 12 and 16 hours p.i., cells were fixed and stained for NP (shown in red color), and also for the NS1 protein at the 16 h time-point (shown in green color). Microscopic sample analysis was conducted by confocal laser scanning microscopy.)
Mouse Influenza B Monoclonal Antibody
Influenza B, Lee 40, Singapore 222/79, Nucleoprotein (MaxLight 650)
Applications
Immunofluorescence, Western Blot
Purity
Purified by Affinity Chromatography from tissue culture supernatant.
Synonyms
Influenza B; Monoclonal Antibody; Lee 40; Singapore 222/79; Nucleoprotein (MaxLight 650); anti-Influenza B antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3H2463
Specificity
Recognizes an epitope within the Influenza B nucleoprotein.
Purity/Purification
Purified by Affinity Chromatography from tissue culture supernatant.
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight650.
Applicable Applications for anti-Influenza B antibody
FLISA, Immunofluorescence (IF), Western Blot (WB)
Immunogen
Influenza B/Lee/40 and B/Singapore/-222/79 viruses
Conjugate
MaxLight650
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Western Blot (WB)
(I7651-58A used for the evaluation of viral nucleoprotien by immunofluorescence and western blot. The nuclear egress of vRNPs late in infection leads to PKR activation. (A) A549 cells grown on glass cover slips were infected with WT or mutant virus #4 at an MOI of 1. Cells were mock treated or complemented with LMB starting at 3 hours p.i.. At 8, 12 and 16 hours p.i., cells were fixed and stained for NP (shown in red color), and also for the NS1 protein at the 16 h time-point (shown in green color). Microscopic sample analysis was conducted by confocal laser scanning microscopy.)
Western Blot (WB)
(I7651-58A used for the evaluation of viral nucleoprotien by immunofluorescence and western blot. The nuclear egress of vRNPs late in infection leads to PKR activation. (A) A549 cells grown on glass cover slips were infected with WT or mutant virus #4 at an MOI of 1. Cells were mock treated or complemented with LMB starting at 3 hours p.i.. At 8, 12 and 16 hours p.i., cells were fixed and stained for NP (shown in red color), and also for the NS1 protein at the 16 h time-point (shown in green color). Microscopic sample analysis was conducted by confocal laser scanning microscopy.)
Product Categories/Family for anti-Influenza B antibody
References
1. Walls, H. H. et al. (1986) Characterization and evalution of monoclonal antibodies developed for typing Influenza A and Influenza B viruses. J. Clin. Micro. 23: 240-245. 2. Zhirnov, O. P. et al. (1999) Caspase-dependent N-terminal cleavage of Influenza virus nucleocapsid protein in infected cells J. Virol. 73: 10158-10163. 3. Ehrhardt, C. et al. (2007) Activation of Phosphatidylinositol 3-Kinase signaling by the nonstructuralNS1 protein is not conserved among Type A and B Influenza viruses. J. Virol. 81: 12097-12100. 4. Goujon, C. et al. (2010) Characterization of the Alpha Interferon-Induced Postentry Block to HIV-1 Infection in Primary Human Macrophages and T Cells. J Virol. 84: 9254-66. 5. Dauber, B. et al. (2009) Influenza B virus ribonucleoprotein is a potent activator of the antiviral kinase PKR. PLoS Pathog. 5:e1000473.
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Product Notes
The Influenza B (Catalog #AAA6256072) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Influenza B can be used in a range of immunoassay formats including, but not limited to, FLISA, Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the Influenza B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Influenza B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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