Mouse anti-Human FADD Monoclonal Antibody | anti-fadd antibody
Mouse Anti-Human FADD-SEPH
Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Synonyms
FADD; Monoclonal Antibody; Mouse Anti-Human FADD-SEPH; Mouse Anti-Human FADD - Sepharose-Labeled; Mouse Anti-Human FADD; anti-fadd antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
1A11
Specificity
Human FADD
Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1 beta -converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation.1-6
Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1 beta -converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation.1-6
Form/Format
Sepharose 4B (SEPH) Conjugate
Sequence Length
218
Applicable Applications for anti-fadd antibody
ELISA; Immunoprecipitation; Western Blot
Characterization
To insure lot-to-lot consistency, each batch of product is tested by western blotting and/or ELISA to conform with the characteristics of a standard reference reagent.
Preparation and Storage
The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The Sepharose 4B (SEPH) conjugate is supplied as 50 tests in 2 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. Shake well before use.
Reagents are stable for the period shown on the label if stored as directed.
The Sepharose 4B (SEPH) conjugate is supplied as 50 tests in 2 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. Shake well before use.
Reagents are stable for the period shown on the label if stored as directed.
References
1. Chinnaiyan, A.M., K. O'Rourke, M. Tewari, and V.M. Dixit. 1995. Cel. 81:505.
2. Cleveland, J.L., and J.N. Ihle. 1995. Cell 81:479.
3. Muzio, M., A.M. Chinnaiyan, F.C. Kishkel, K. O'Rourke, A. Shevchenko, J. Ni, C. Scaffidi, J.D. Bretz, M. Zhang, R. Gentz, M. Mann, P.H. Krammer, M.E. Peter, and V.M. Dixit. 1996. Cell 85:817.
4. Wieder, T., F. Essmann, A. Prokop, K. Schmelz, K. Schulze-Osthoff, R. Beyaert, B. Dorken, and P.T. Daniel. 2001. Blood 97:1378.
5. Newton, K., A.W. Harris, M.L. Bath, K.G.C. Smith, and A. Strasser. 1998. EMBO J. 17:706.
6. Boldin, M.P., E.E. Varfolomeev, Z. Pancer, I.L. Meet, J.H. Camonis, and D. Wallach. 1995. J. Biol. Chem. 270:7795.
2. Cleveland, J.L., and J.N. Ihle. 1995. Cell 81:479.
3. Muzio, M., A.M. Chinnaiyan, F.C. Kishkel, K. O'Rourke, A. Shevchenko, J. Ni, C. Scaffidi, J.D. Bretz, M. Zhang, R. Gentz, M. Mann, P.H. Krammer, M.E. Peter, and V.M. Dixit. 1996. Cell 85:817.
4. Wieder, T., F. Essmann, A. Prokop, K. Schmelz, K. Schulze-Osthoff, R. Beyaert, B. Dorken, and P.T. Daniel. 2001. Blood 97:1378.
5. Newton, K., A.W. Harris, M.L. Bath, K.G.C. Smith, and A. Strasser. 1998. EMBO J. 17:706.
6. Boldin, M.P., E.E. Varfolomeev, Z. Pancer, I.L. Meet, J.H. Camonis, and D. Wallach. 1995. J. Biol. Chem. 270:7795.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
FADD
NCBI Official Symbol
fadd
NCBI Protein Information
protein FADD
Protein Family
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Product Notes
The fadd (Catalog #AAA670128) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human FADD-SEPH reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FADD can be used in a range of immunoassay formats including, but not limited to, ELISA; Immunoprecipitation; Western Blot. Researchers should empirically determine the suitability of the fadd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FADD, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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