Mouse anti-Human CD95 (Fas/Apo-1) Monoclonal Antibody

Mouse Anti-Human CD95/Fas-FITC

Cat. Num. AAA674299

• Reactivity: Human
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: CD95 (Fas/Apo-1); Monoclonal Antibody; Mouse Anti-Human CD95/Fas-FITC; Mouse Anti-Human CD95/Fas - FITC; Mouse Anti-Human CD95 (Fas/Apo-1); anti-CD95 (Fas/Apo-1) antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: DX3
• Specificity: Human CD95/Fas/Apo-1; CD95, also known as Fas and Apo-1, is a 40-50 kDa type I transmembrane glycoprotein and a member of the tumor necrosis factor receptor superfamily. It is expressed by activated lymphocytes, monocytes, neutrophils, fibroblasts and cell lines. Fas ligand binding to CD95 induces apoptosis in activated mature lymphocytes thereby playing a role in maintaining peripheral tolerance. Crosslinking of CD95 by the monoclonal antibodies DX2 and DX3 delivers an apoptotic signal to Fas-sensitive cells, indicating that these monoclonal antibodies recognize a functional epitope of CD95.1-5
• Form/Format: Fluorescein (FITC) Conjugate

• Applicable Applications for anti-CD95 (Fas/Apo-1) antibody: Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
• Application Notes: Research Application(s): In vitro induction of apoptosis; ; Flow Cytometry Suggested Dilution: 10 uL/10^6 cells
• Characterization: To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The Spectral Red (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of PBS. Aliquot and store at or below -20 degree C. ; Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Testing Data

References

1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd,eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Kishimoto, T., A.E.G. von dern Borne, S.M. Goyert, D.Y. Mason, M. Miyasaka, L. Moretta, K. Okumura, S. Shaw, T.A. Springer, K.Sugamura, and H. Zola, eds. 1998. Leukocyte Typing VI: White Cell Differentiation Antigens, Academic Press, New York.
3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD95 Section, Academic Press, New York, p. 363.
4. Nagata, S., and P. Golstein. 1995. Science 267:3378.
5. van Parijs, L., and A.K. Abbas. 1996. Curr. Opin. Immunol. 8:355.