Rabbit anti-Human MXD1 Polyclonal Antibody | anti-MXD1 antibody

MXD1, NT (MXD1, MAD, Max dimerization protein 1, Protein MAD) (APC)

Cat. Num. AAA6323078

• Gene Names: MXD1; MAD; MAD1; BHLHC58; MGC104659
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MXD1; Polyclonal Antibody; NT (MXD1; MAD; Max dimerization protein 1; Protein MAD) (APC); Protein MAD; anti-MXD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MXD1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MXD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-50 amino acids from the N-terminal region of human MXD1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MXD1 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MXD1 antibody detected the MXD1 protein (arrow).)

Related Product Information for anti-MXD1 antibody

MAX dimerization protein belongs to a subfamily of MAX-interacting proteins. This protein competes with MYC for binding to MAX to form a sequence-specific DNA-binding complex, acts as a transcriptional repressor (while MYC appears to function as an activator) and is a candidate tumor suppressor. [provided by RefSeq].

Product Categories/Family for anti-MXD1 antibody

Antibodies; Transcription Factors

References

Wu, S., et al. Exp. Cell Res. 315(18):3099-3111(2009). Chou, C.K., et al. Mol. Carcinog. 48(11):1048-1058(2009). Zhu, J., et al. Proc. Natl. Acad. Sci. U.S.A. 105(18):6584-6589(2008). Jiang, K., et al. Nucleic Acids Res. 36(5):1517-1531(2008). Suo, X.H., et al. Zhonghua Xue Ye Xue Za Zhi 28(11):745-749(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4505069
• NCBI GeneID: 4084
• NCBI Accession #: NP_002348.1
• NCBI GenBank Nucleotide #: NM_002357.3
• UniProt Accession #: Q05195; Q6FI41; B2R6V8; D6W5G2
• Molecular Weight: 25,254 Da
• NCBI Official Full Name: max dimerization protein 1 isoform 1
• NCBI Official Synonym Full Names: MAX dimerization protein 1
• NCBI Official Symbol: MXD1
• NCBI Official Synonym Symbols: MAD; MAD1; BHLHC58; MGC104659
• NCBI Protein Information: max dimerization protein 1; max dimerizer 1; OTTHUMP00000160049; MAX-binding protein; antagonizer of myc transcriptional activity
• UniProt Protein Name: Max dimerization protein 1
• Protein Family: Max dimerization protein
• UniProt Gene Name: MXD1
• UniProt Synonym Gene Names: MAD; Max dimerizer 1
• UniProt Entry Name: MAD1_HUMAN

NCBI Description

This gene encodes a member of the MYC/MAX/MAD network of basic helix-loop-helix leucine zipper transcription factors. The MYC/MAX/MAD transcription factors mediate cellular proliferation, differentiation and apoptosis. The encoded protein antagonizes MYC-mediated transcriptional activation of target genes by competing for the binding partner MAX and recruiting repressor complexes containing histone deacetylases. Mutations in this gene may play a role in acute leukemia, and the encoded protein is a potential tumor suppressor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq]

Uniprot Description

Mad1: Transcriptional repressor. MAD binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MAD thus antagonizes MYC transcriptional activity by competing for MAX. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with RNF17. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2p13-p12

Cellular Component: nucleus

Molecular Function: protein dimerization activity; transcription cofactor activity; transcription corepressor activity; transcription factor activity

Biological Process: cell proliferation; transcription, DNA-dependent; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter