Mouse anti-Human FAC1 / BPTF Monoclonal Antibody | anti-FAC1 antibody

Mouse Monoclonal [clone 2F10] (IgG2b) to Human FAC1 / BPTF

Cat. Num. AAA249006

• Gene Names: BPTF; FAC1; FALZ; NURF301
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Ascites
• Synonyms: FAC1 / BPTF; Monoclonal Antibody; Mouse Monoclonal [clone 2F10] (IgG2b) to Human FAC1 / BPTF; Anti-FAC1 / BPTF Antibody (clone 2F10) IHC-plus; BPTF; FAC1; FALZ; Fetal Alz-50 clone 1 protein; Fetal Alz-50 reactive clone 1; Fetal Alzheimer antigen; NURF301; Human FAC1; anti-FAC1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 2F10
• Specificity: Human FAC1 / BPTF
• Purity/Purification: Ascites
• Form/Format: Ascites, 0.03% sodium azide
• Sequence Length: 2903

• Applicable Applications for anti-FAC1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IHC-P (1:50), WB (1:500 - 1:2000)
• Target Species: Human
• Immunogen Description: Purified recombinant fragment of human BPTF expressed in E Coli.
• Immunogen Type: Recombinant protein
• Immunogen: FAC1 / BPTF antibody was raised against purified recombinant fragment of human BPTF expressed in E Coli.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-FAC1 / BPTF antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

Western Blot (WB)

(Western blot of BPTF mAb against HEK293 (1) and BPTF (AA: 503-670)-hIgGFc transfected HEK293 (2) cell lysate.)

ELISA (EIA)

(Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)

NCBI and Uniprot Product Information

• NCBI GI #: 38788260
• NCBI GeneID: 2186
• NCBI Accession #: NP_004450.3
• NCBI GenBank Nucleotide #: NM_004459.6
• UniProt Accession #: Q12830; Q6NX67; Q7Z7D6; Q9UIG2
• Molecular Weight: 322,217 Da
• NCBI Official Full Name: nucleosome-remodeling factor subunit BPTF isoform 2
• NCBI Official Synonym Full Names: bromodomain PHD finger transcription factor
• NCBI Official Symbol: BPTF
• NCBI Official Synonym Symbols: FAC1; FALZ; NURF301
• NCBI Protein Information: nucleosome-remodeling factor subunit BPTF; fetal Alzheimer antigen; fetal Alz-50 clone 1 protein; fetal Alz-50 reactive clone 1; nucleosome remodeling factor, large subunit; bromodomain and PHD domain transcription factor; bromodomain and PHD finger-containing transcription factor
• UniProt Protein Name: Nucleosome-remodeling factor subunit BPTF
• UniProt Gene Name: BPTF
• UniProt Synonym Gene Names: FAC1; FALZ
• UniProt Entry Name: BPTF_HUMAN

NCBI Description

This gene was identified by the reactivity of its encoded protein to a monoclonal antibody prepared against brain homogenates from patients with Alzheimer's disease. Analysis of the original protein (fetal Alz-50 reactive clone 1, or FAC1), identified as an 810 aa protein containing a DNA-binding domain and a zinc finger motif, suggested it might play a role in the regulation of transcription. High levels of FAC1 were detected in fetal brain and in patients with neurodegenerative diseases. The protein encoded by this gene is actually much larger than originally thought, and it also contains a C-terminal bromodomain characteristic of proteins that regulate transcription during proliferation. The encoded protein is highly similar to the largest subunit of the Drosophila NURF (nucleosome remodeling factor) complex. In Drosophila, the NURF complex, which catalyzes nucleosome sliding on DNA and interacts with sequence-specific transcription factors, is necessary for the chromatin remodeling required for transcription. Two alternative transcripts encoding different isoforms have been described completely. [provided by RefSeq, Jul 2008]

Uniprot Description

FALZ: Histone-binding component of NURF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. May also regulate transcription through direct binding to DNA or transcription factors. Belongs to the PBTF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q24.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: DNA-dependent ATPase activity; protein binding; zinc ion binding; sequence-specific DNA binding; transcription factor binding

Biological Process: anterior/posterior pattern formation; chromatin remodeling; embryonic placenta development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; brain development; negative regulation of transcription from RNA polymerase II promoter; endoderm development