Chromatin Immunoprecipitation (ChIP)
(Suz12 antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and HeLa chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of Suz12 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the Myt1 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Mouse anti-Human Suz12 Monoclonal Antibody | anti-Suz12 antibody
Suz12 antibody (mAb)(Clone 2AO9)
ChIP: 2.5-10ug per ChIP
This antibody has been published, but has not been validated, for Western blot. For an antibody validated for use in Western blotting
Chromatin Immunoprecipitation (ChIP)
(Suz12 antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and HeLa chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of Suz12 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the Myt1 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Chromatin Immunoprecipitation (ChIP)
(Suz12 antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and HeLa chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of Suz12 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the Myt1 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Background: Suz12 (Suppressor-of-Zeste 12 protein, also designated as ChET9, JJAZ1 protein) is a component of the Polycomb group complexes 2, 3 and 4 (PRC2, PRC3 and PRC4), which also contain the histone methyltransferase EZH2, EED (Embryonic Ectoderm Development protein) protein and the histone-binding proteins RbAP46 and RbAP48. PRC2 is responsible for di- and trimethylation of histone 3 at lysine 27 (Histone H3K27me2 and Histone H3K27me3), which leads to transcriptional repression of the target genes. Suz12 is a nuclear protein belonging to the VEFS family. Suz12 contains a zinc-finger binding motif and a VEFS box, which is required for the interaction between Suz12 and EZH2.
NCBI and Uniprot Product Information
Observed: 96 kDa
NCBI Description
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq, Jul 2009]
Uniprot Description
SUZ12: a zinc finger protein. A breakpoint translocation of the cognate gene with JAZF1 has been reported in endometrial stromal sarcoma.
Protein type: Oncoprotein; C2H2-type zinc finger protein; Transcription factor
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: nucleoplasm; sex chromatin; ESC/E(Z) complex; nucleolus; nucleus
Molecular Function: histone methyltransferase activity; protein binding; sequence-specific DNA binding; RNA binding; metal ion binding; chromatin binding; methylated histone residue binding
Biological Process: histone methylation; negative regulation of cell differentiation; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; histone ubiquitination; positive regulation of cell proliferation; gene expression; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic
Research Articles on Suz12
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Product Notes
The Suz12 suz12 (Catalog #AAA388616) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Suz12 antibody (mAb)(Clone 2AO9) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Suz12 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Immunoprecipitation (IP). Applications Validated: ChIP: 2.5-10ug per ChIP This antibody has been published, but has not been validated, for Western blot. For an antibody validated for use in Western blotting. Researchers should empirically determine the suitability of the Suz12 suz12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Suz12, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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