Rabbit anti-Mouse Insulin Like Growth Factor 1 (IGF1) Polyclonal Antibody | anti-IGF1 antibody

Polyclonal Antibody to Insulin Like Growth Factor 1 (IGF1)

Cat. Num. AAA2004492

• Gene Names: Igf1; Igf-1; Igf-I; C730016P09Rik
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Insulin Like Growth Factor 1 (IGF1); Polyclonal Antibody; Polyclonal Antibody to Insulin Like Growth Factor 1 (IGF1); anti-IGF1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against IGF1. It has been selected for its ability to recognize IGF1 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with a His-tag and its sequence is listed below.; MGHHHHHHSGSEF-GPETLCGA ELVDALQFVC GPRGFYFNKP TGYGSSIRRA PQTGIVDECC FRSCDLRRLE MYCAPLKPTK AA
• Sequence Length: 154

• Applicable Applications for anti-IGF1 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant IGF1 (Gly33~Ala102) expressed in E.coli.
• Cross Reactivity: Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2036103
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant IGF1, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded liver tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 163659889
• NCBI GeneID: 16000
• NCBI Accession #: NP_001104744.1
• NCBI GenBank Nucleotide #: NM_001111274.1
• UniProt Accession #: Q8CAR0; P05017
• Molecular Weight: 18,473 Da
• NCBI Official Full Name: insulin-like growth factor I isoform 3
• NCBI Official Synonym Full Names: insulin-like growth factor 1
• NCBI Official Symbol: Igf1
• NCBI Official Synonym Symbols: Igf-1; Igf-I; C730016P09Rik
• NCBI Protein Information: insulin-like growth factor I
• UniProt Protein Name: Insulin-like growth factor I
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: Igf1

NCBI Description

This gene encodes a member of the insulin-like growth factor (IGF) family of proteins that promote growth and development during fetal and postnatal life. This gene is predominantly expressed in the liver and the encoded protein undergoes proteolytic processing to generate a disulfide-linked mature polypeptide. Transgenic disruption of this gene in mice results in reduced postnatal survival and severe growth retardation. Mice lacking the encoded protein exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]