Testing Data
(Fig. LEFT: 1:1,000 (1ug/mL) Ab dilution used in WB of HEK293 cell lysate; 5ug (1), 10ug (2), and 30ug (3) of cell lysate used; RIGHT: IP of anti-ERK1 (1uL) using HeLa cell lysate; 10ug (1), 25ug (2), and 50ug (3) of cell lysate used)
Mouse ERK1 Monoclonal Antibody | anti-ERK1 antibody
Anti-ERK1 (E32) Signal Transduction Monoclonal Antibody
Applications
Western Blot, Immunoprecipitation
Purity
Protein A affinity chromatography from mouse ascites fluid
Synonyms
ERK1; Monoclonal Antibody; Anti-ERK1 (E32) Signal Transduction Monoclonal Antibody; Anti-ERK1; anti-ERK1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
E32
Specificity
Recognizes only the ERK1 protein (e.g.: from human and mouse cells)
Purity/Purification
Protein A affinity chromatography from mouse ascites fluid
Form/Format
Liquid; 1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3
Sequence Length
422
Applicable Applications for anti-ERK1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature)
IP: 1:50-200
For best results with other assays, please determine optimal working dilution by titration test.
IP: 1:50-200
For best results with other assays, please determine optimal working dilution by titration test.
Immunogen
Recombinant full length ERK1 protein
Cross Reactivity
WB: ERK1 from human and mouse cells. IP: ERK1 from human cells. ERK1 from other species may also be detectable
Preparation and Storage
Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Testing Data
(Fig. LEFT: 1:1,000 (1ug/mL) Ab dilution used in WB of HEK293 cell lysate; 5ug (1), 10ug (2), and 30ug (3) of cell lysate used; RIGHT: IP of anti-ERK1 (1uL) using HeLa cell lysate; 10ug (1), 25ug (2), and 50ug (3) of cell lysate used)
Testing Data
(Fig. LEFT: 1:1,000 (1ug/mL) Ab dilution used in WB of HEK293 cell lysate; 5ug (1), 10ug (2), and 30ug (3) of cell lysate used; RIGHT: IP of anti-ERK1 (1uL) using HeLa cell lysate; 10ug (1), 25ug (2), and 50ug (3) of cell lysate used)
Product Categories/Family for anti-ERK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Extracellular signal-regulated kinase 1
NCBI Official Symbol
ERK1
NCBI Protein Information
MAP kinase-like orf; aka CEK1; one of two protein kinase genes similar to S. cerevisiae MAP kinases FUS3 (YBL016W) and KSS1 (YGR040W) involved in pheromone signal transduction; likely protein kinase
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Product Notes
The ERK1 (Catalog #AAA525012) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ERK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature) IP: 1:50-200 For best results with other assays, please determine optimal working dilution by titration test. Researchers should empirically determine the suitability of the ERK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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