Rabbit anti-Mouse SP2 Polyclonal Antibody | anti-SP2 antibody

SP2, CT (SP2, KIAA0048, Transcription factor Sp2) (PE)

Cat. Num. AAA6350183

• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SP2; Polyclonal Antibody; CT (SP2; KIAA0048; Transcription factor Sp2) (PE); Transcription factor Sp2; anti-SP2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SP2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 497-527 amino acids from the C-terminal region of human SP2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SP2 Antibody (C-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the SP2 antibody detected the SP2 protein (arrow).)

Related Product Information for anti-SP2 antibody

This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters.

Product Categories/Family for anti-SP2 antibody

Antibodies; Transcription Factors

References

Ito, H., et al. Biochim. Biophys. Acta 1789 (11-12), 681-690 (2009): Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009): Letourneur, M., et al. Mol. Immunol. 46 (11-12), 2151-2160 (2009): Sun, J.M., et al. J. Biol. Chem. 282(45):33227-33236(2007). Das, A., et al. J. Biol. Chem. 281(51):39105-39113(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 125625357
• NCBI GeneID: 6668
• NCBI Accession #: NP_003101.3
• NCBI GenBank Nucleotide #: NM_003110.5
• UniProt Accession #: Q02086; A6NK74
• Molecular Weight: 64,900 Da
• NCBI Official Full Name: transcription factor Sp2
• NCBI Official Synonym Full Names: Sp2 transcription factor
• NCBI Official Symbol: SP2
• NCBI Protein Information: transcription factor Sp2
• UniProt Protein Name: Transcription factor Sp2
• Protein Family: Sex-specific storage-protein
• UniProt Gene Name: SP2
• UniProt Synonym Gene Names: KIAA0048
• UniProt Entry Name: SP2_HUMAN

NCBI Description

This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. [provided by RefSeq, Jul 2008]

Uniprot Description

SP2: Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Belongs to the Sp1 C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; nucleus

Molecular Function: DNA binding; histone deacetylase binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; fibroblast proliferation; in utero embryonic development; multicellular organism growth; embryonic organ development; immune response