Mouse anti-Human ErbB4 Monoclonal Antibody | anti-Erbb4 antibody

ErbB4 (c-erbB4, HER4)

Cat. Num. AAA603494

• Gene Names: Erbb4; Her4; c-erbB-4
• Reactivity: Human
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: ErbB4; Monoclonal Antibody; ErbB4 (c-erbB4; HER4); Anti -ErbB4 (c-erbB4; anti-Erbb4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3F166
• Specificity: Recognizes human c-erbB-4/HER-4 oncoprotein. Species Crossreactivity: Human, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See E3451-40AX

• Applicable Applications for anti-Erbb4 antibody: Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, Immunoprecipitation, Internalization of c-erbB-4 and stimulation of tyrosine kinase activity. Not suitable for use in Western Blot and Inhibition of receptor-heregulin binding1.; Dilution: Immunoprecipitation: 2ug/mg protein lysate (Use Protein G). (Unoccupied as well as the occupied c-erbB-4 receptor).
• Immunogen: Extracellular fragment of recombinant human c-erbB-4/HER-4 oncoprotein
• Cellular Localization: Cell membrane and cytoplasmic
• Positive Control: T47D or MDA-MB-453 cells
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C or colder. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Erbb4 antibody

c-erbB-4 is the fourth member of class I receptor kinase family (ErbB). Down regulation of ErbB-4 in estrogen receptor-positive cancer cells inhibits proliferation.

Product Categories/Family for anti-Erbb4 antibody

Antibodies; Abs to Oncoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 986917
• NCBI GeneID: 13869
• UniProt Accession #: O88459; Q61527
• Molecular Weight: 185kD
• NCBI Official Full Name: erbB4
• NCBI Official Synonym Full Names: v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
• NCBI Official Symbol: Erbb4
• NCBI Official Synonym Symbols: Her4; c-erbB-4
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-4; OTTMUSP00000030425; OTTMUSP00000030426; OTTMUSP00000030427; avian erythroblastosis oncogene B 4; proto-oncogene-like protein c-ErbB-4
• UniProt Protein Name: EGF-like growth factor receptor ErbB4 extracellular domain
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: Erbb4
• UniProt Entry Name: O88459_MOUSE

Uniprot Description

HER4: a receptor tyrosine kinase of the EGFR family. Specifically binds and is activated by neuregulins, NRG- 2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Heterodimerizes and signals with other EGF receptors. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with WWOX. May act as a tumor suppressor: overexpressed in head and neck cancer , but downregulated in renal cancer, papillary carcinoma, high-grade gliomas and invasive breast cancer. 3 alternatively-spliced isoforms of the human protein have been reported.

Protein type: Kinase, protein; Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; TK group; EGFR family

Cellular Component: mitochondrion; membrane; basolateral plasma membrane; cytoplasm; postsynaptic density; plasma membrane; integral to membrane; caveola; nucleus; receptor complex; lipid raft

Molecular Function: transferase activity; protein binding; protein homodimerization activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; epidermal growth factor receptor binding; protein kinase activity

Biological Process: lactation; synapse maturation; peptidyl-tyrosine phosphorylation; apoptosis; heart development; multicellular organismal development; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; embryonic pattern specification; signal transduction; olfactory bulb interneuron differentiation; protein amino acid phosphorylation; regulation of cell migration; positive regulation of glucose import; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; neural crest cell migration; positive regulation of cardiac muscle cell proliferation; nervous system development; cell migration; cell fate commitment; transcription, DNA-dependent; positive regulation of synaptic transmission, GABAergic; central nervous system morphogenesis; positive regulation of tyrosine phosphorylation of Stat5 protein; mitochondrial fragmentation during apoptosis; phosphorylation; surfactant homeostasis; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of epithelial cell proliferation; positive regulation of cell migration; negative regulation of apoptosis