Immunohistochemistry (IHC)
(Immunohistochemistry using MBS601288.)
Mouse anti-Human, Rat c-erbB2, phosphospeci?c Monoclonal Antibody
c-erbB2, phosphospeci?c (HER-2, neu Receptor) (BSA & Azide Free)
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
c-erbB2; phosphospeci?c; Monoclonal Antibody; phosphospeci?c (HER-2; neu Receptor) (BSA & Azide Free); Anti -c-erbB2; phosphospeci c (HER-2; anti-c-erbB2; phosphospeci?c antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3F38
Specificity
Highly specific to human activated, tyrosine-phosphorylated (pTyr1248) form of c-erbB-2. Does not crossreact with closely related receptors such as EGFR, HER-3 or HER-4.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C0026-14.
Applicable Applications for anti-c-erbB2, phosphospeci?c antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry (Formalin/paraffin): 10-20ug/ml for 2hrs at RT. Note: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Dilution: Immunohistochemistry (Formalin/paraffin): 10-20ug/ml for 2hrs at RT. Note: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Immunogen
Tyrosine-phosphorylated synthetic peptide corresponding to aa1242-1255 (Lys-TAENPE-pY-LGLDVPV) from the C-terminus of human c-erbB-2 protein (pTyr1248). Cellular Localization: Cell membrane. MW of Antigen: 185kD
Positive Control
EGF treated SKBR-3 cells. About 5% of breast carcinomas are positive for phospho-c-erbB-2/HER-2/neu oncoprotein.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Immunohistochemistry using MBS601288.)
Immunohistochemistry (IHC)
(Immunohistochemistry using MBS601288.)
Related Product Information for anti-c-erbB2, phosphospeci?c antibody
c-erbB-2 is a receptor tyrosine kinase of the c-erbB family. Approximately 25% of primary breast and ovarian tumors were found to overexpress the protein.
Product Categories/Family for anti-c-erbB2, phosphospeci?c antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Chain P, Single Chain Fv Of C219 Heavy Chain V101l Mutant In Complex With Synthetic Epitope Peptide
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Product Notes
The c-erbB2, phosphospeci?c (Catalog #AAA601288) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The c-erbB2, phosphospeci?c (HER-2, neu Receptor) (BSA & Azide Free) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's c-erbB2, phosphospeci?c can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Dilution: Immunohistochemistry (Formalin/paraffin): 10-20ug/ml for 2hrs at RT. Note: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the c-erbB2, phosphospeci?c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-erbB2, phosphospeci?c, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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