Mouse anti-Human ELAVL1 / HUR Monoclonal Antibody | anti-ELAVL1 antibody

Mouse Monoclonal [clone 4G8] (IgG1,k) to Human ELAVL1 / HUR

Cat. Num. AAA246473

• Gene Names: ELAVL1; HUR; Hua; MelG; ELAV1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: ELAVL1 / HUR; Monoclonal Antibody; Mouse Monoclonal [clone 4G8] (IgG1; k) to Human ELAVL1 / HUR; Anti-ELAVL1 / HUR Antibody (clone 4G8) IHC-plus; ELAVL1; ELAV1; Hua; HUR; Hu antigen R; ELAV-like protein 1; Hu-antigen R; MelG; Human ELAVL1; anti-ELAVL1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4G8
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.42 mg/ml (varies by lot)
• Sequence Length: 326

• Applicable Applications for anti-ELAVL1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (10 ug/ml), WB; Usage: Western Blot (Cell lysate) - positive control HeLa. Immunofluorescence (10 ug/ml) - positive control HeLa cells.
• Target Species: Human
• Immunogen Description: ELAVL1 (AAH03376, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: ELAVL1 / HUR antibody was raised against eLAVL1 (AAH03376, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-HuR antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-HuR antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-HuR antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ELAVL1 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(ELAVL1 monoclonal antibody clone 4G8 Western blot of ELAVL1 expression in HeLa.)

Related Product Information for anti-ELAVL1 antibody

A member of the ELAVL protein family, ELAVL1 contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression.

NCBI and Uniprot Product Information

• NCBI GI #: 38201714
• NCBI GeneID: 1994
• NCBI Accession #: NP_001410.2
• NCBI GenBank Nucleotide #: NM_001419.2
• UniProt Accession #: Q15717; Q53XN6; Q9BTT1; B4DVB8
• Molecular Weight: 38,996 Da
• NCBI Official Full Name: ELAV-like protein 1
• NCBI Official Synonym Full Names: ELAV like RNA binding protein 1
• NCBI Official Symbol: ELAVL1
• NCBI Official Synonym Symbols: HUR; Hua; MelG; ELAV1
• NCBI Protein Information: ELAV-like protein 1; Hu antigen R; hu-antigen R; embryonic lethal, abnormal vision, drosophila, homolog-like 1; ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• UniProt Protein Name: ELAV-like protein 1
• Protein Family: ELAV-like protein
• UniProt Gene Name: ELAVL1
• UniProt Synonym Gene Names: HUR; HuR
• UniProt Entry Name: ELAV1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. [provided by RefSeq, Sep 2012]

Uniprot Description

HuR: Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A, EIF2C1 and EIF2C2. Ubiquitous. Belongs to the RRM elav family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus; cytosol

Molecular Function: mRNA binding; protein binding; mRNA 3'-UTR binding; RNA binding; double-stranded RNA binding; nucleotide binding; AU-rich element binding; protein kinase binding

Biological Process: positive regulation of translation; mRNA stabilization; multicellular organismal development; gene expression