Mouse anti-Human Elafin Monoclonal Antibody | anti-PI3 antibody

Elafin, Human, mAb TRAB2O

Cat. Num. AAA584017

• Gene Names: PI3; ESI; WAP3; SKALP; WFDC14; cementoin
• Reactivity: Human
• Applications: Western Blot
• Synonyms: Elafin; Monoclonal Antibody; Human; mAb TRAB2O; anti-PI3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 117

• Applicable Applications for anti-PI3 antibody: Immunoassay (IA), P, Western Blot (WB)
• Application Notes: Application Use: For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For Western blotting it is recommended to use non-reducing conditions.
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-PI3 antibody

Product Description: Elafin is an epithelial proteinase inhibitor also known under various other names such as Skin-derived Anti leukoproteinase (SKALP) and Elastase-Specific Inhibitor (ESI). Elafin belongs to the Trappin gene family and was given the systematic name Trappin-2. The Trappin family is defined by a N-terminal transglutaminase substrate domain and a C-terminal four disulphide core. Trappins have been suggested to play a role in the regulation of inflammation and in protection against tissue damage in stratified epithelia. Elafin is an inhibitor of leukocyte elastase and proteinase-3 and is a substrate for transglutaminases. The protein is constitutively expressed in various epithelia including hair follicles, oesophagus, vagina and oral cavity. Elafin is not present in normal human skin but is strongly induced during inflammation as in psoriasis and wound healing. Antibodies to Elafin can be used to evaluate the effects of treatment of psoriasis since its expression is significantly correlated with clinical scores. Antibodies to Elafin have also been succesfully used to study differentiation in squamous cell carcinoma of the head-and-neck region, oesophagus and skin. It is also shown that Elafin possesses antimicrobial activity against gram-positive and gram-negative bacteria. TRAB2O recognises all known Trappins via the GQDPVK epitope. TRAB2O shows strong reaction with human native full-length Elafin/SKALP and a weaker reaction with fully processed Elafin/SKALP (C-terminal 57 aa). For detection of fully processed Elafin/SKALP (C-terminal 57 aa) we advise to use HM2063. The antibody is cross reactive with Trappin family members of other species with GQDPVK epitope.

References

1. Vandermeeren, M et al; Development and application of monoclonal antibodies against SKALP/elafin and other trappin family members. Arch Dermatol Res 2001, 293: 343
2. Alkemade, J et al; Demonstration of skin-derived antileukoproteinase (SKALP) and its target enzyme human leukocyte elastase in squamous carcinoma. J Pathol 1994, 174: 121
3. Bergen van, H et al; Expression of SKALP/elafin during wound healing in human skin. Arch Dermatol Res 1996, 288: 458
4. Pfundt, R et al; Constitutive and inducible expression of SKALP/elafin provides anti-elastase defense in human epithelia. J Clin Invest 1996, 98: 1389
5. Schalkwijk, J et al; Localization of SKALP/elafin. J Invest Dermatol 1993, 100: 390
6. Schalkwijk, J et al; The trappin gene family: proteins defined by an N-terminal transglutaminase substrate domain and a c-terminal four-disulphide core. Biochem J 1999, 340: 569
7. Wiedow, O et al; Elafin: an elastase-specific inhibitor of human skin. J Biol Chem 1990, 265: 14791
8. Huh, WK et al; Dynamic alteration of human beta-defensin 2 localization from cytoplasm to intercellular space in psoriatic skin. J Mol Med 2002, 80: 678

NCBI and Uniprot Product Information

• NCBI GI #: 4505787
• NCBI GeneID: 5266
• NCBI Accession #: NP_002629.1
• NCBI GenBank Nucleotide #: NM_002638.3
• NCBI Official Full Name: elafin preproprotein
• NCBI Official Synonym Full Names: peptidase inhibitor 3, skin-derived
• NCBI Official Symbol: PI3
• NCBI Official Synonym Symbols: ESI; WAP3; SKALP; WFDC14; cementoin
• NCBI Protein Information: elafin; PI-3; trappin-2; pre-elafin; protease inhibitor WAP3; elastase-specific inhibitor; skin-derived antileukoproteinase; WAP four-disulfide core domain 14; WAP four-disulfide core domain protein 14; protease inhibitor 3, skin-derived (SKALP)
• UniProt Protein Name: Elafin
• Protein Family: Elafin
• UniProt Gene Name: PI3
• UniProt Synonym Gene Names: WAP3; WFDC14; ESI; PI-3; SKALP
• UniProt Entry Name: ELAF_HUMAN

NCBI Description

This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq, Oct 2008]

Uniprot Description

elafin: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Protein type: Secreted; Secreted, signal peptide; Inhibitor; Extracellular matrix

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: proteinaceous extracellular matrix

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: copulation