Mouse anti-Human Elafin Monoclonal Antibody | anti-PI3 antibody

Elafin (SKALP, ESI, ELAFIN, Elafin precursor, Elastase-specific inhibitor, ESI, MGC13613, Peptidase inhibitor 3, Protease inhibitor WAP3, SKALP, Skin-derived antileukoproteinase, WAP3, WAP four-disulfide core domain protein 14, WFDC14)

Cat. Num. AAA605154

• Gene Names: PI3; ESI; WAP3; SKALP; WFDC14; MGC13613; cementoin
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified
• Synonyms: Elafin; Monoclonal Antibody; Elafin (SKALP; ESI; ELAFIN; Elafin precursor; Elastase-specific inhibitor; MGC13613; Peptidase inhibitor 3; Protease inhibitor WAP3; SKALP; Skin-derived antileukoproteinase; WAP3; WAP four-disulfide core domain protein 14; WFDC14); Anti -Elafin (SKALP; anti-PI3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9G181
• Specificity: Recognizes human native full-length Elafin. Weakly recognizes fully processed Elafin (C-terminal 57aa). Recognizes all known Trappins via the GQDPVK epitope. Cross reacts with Trappin family members of other species with GQDPVK epitope. For detection of fully processed Elafin (C-terminal 57aa) use E2225-01.
• Purity/Purification: Affinity Purified; Purified
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

• Applicable Applications for anti-PI3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:10 Non-reduced conditions recommended.; Immunohistochemistry (paraffin): 1:10
• Immunogen: Human Elafin/SKALP
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-PI3 antibody

Elafin is an epithelial proteinase inhibitor also known under various other names such as Skin-derived Anti leukoproteinase (SKALP) and Elastase-Specific Inhibitor (ESI). Elafin belongs to the Trappin gene family and was given the systematic name Trappin-2. The Trappin family is defined by a N-terminal transglutaminase substrate domain and a C-terminal four disulfide core. Trappins have been suggested to play a role in the regulation of inflammation and in protection against tissue damage in stratified epithelia. Elafin is an inhibitor of leukocyte elastase and proteinase-3 and is a substrate for transglutaminases. The protein is constitutively expressed in various epithelia including hair follicles, oesophagus, vagina and oral cavity. Elafin is not present in normal human skin but is strongly induced during inflammation as in psoriasis and wound healing. Antibodies to Elafin can be used to evaluate the effects of treatment of psoriasis since its expression is significantly correlated with clinical scores. Antibodies to Elafin have also been succesfully used to study differentiation in squamous cell carcinoma of the head-and-neck region, oesophagus and skin. It is also shown that Elafin possesses antimicrobial activity against gram-positive and gram-negative bacteria.

Product Categories/Family for anti-PI3 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4505787
• NCBI GeneID: 5266
• NCBI Accession #: NP_002629.1
• NCBI GenBank Nucleotide #: NM_002638.3
• UniProt Accession #: P19957; Q6FG74; E1P618; Q6LD69
• Molecular Weight: 12,270 Da
• NCBI Official Full Name: elafin preproprotein
• NCBI Official Synonym Full Names: peptidase inhibitor 3, skin-derived
• NCBI Official Symbol: PI3
• NCBI Official Synonym Symbols: ESI; WAP3; SKALP; WFDC14; MGC13613; cementoin
• NCBI Protein Information: elafin; PI-3; trappin-2; pre-elafin; OTTHUMP00000031100; protease inhibitor WAP3; elastase-specific inhibitor; skin-derived antileukoproteinase; WAP four-disulfide core domain 14; WAP four-disulfide core domain protein 14; protease inhibitor 3, skin-derived (SKALP)
• UniProt Protein Name: Elafin
• Protein Family: Elafin
• UniProt Gene Name: PI3
• UniProt Synonym Gene Names: WAP3; WFDC14
• UniProt Entry Name: ELAF_HUMAN

NCBI Description

This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq]

Uniprot Description

elafin: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix; Inhibitor

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: proteinaceous extracellular matrix

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: copulation