Human Poliovirus Receptor (PVR) ELISA Kit | PVR elisa kit

Human Poliovirus Receptor (PVR) ELISA Kit

Cat. Num. AAA2023013

• Gene Names: PVR; PVS; HVED; CD155; NECL5; TAGE4; Necl-5
• Reactivity: Human
• Synonyms: Poliovirus Receptor (PVR); Human Poliovirus Receptor (PVR) ELISA Kit; CD155; HVED; Necl-5; Tage4; Nectin-Like Protein 5; PVR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Poliovirus Receptor (PVR).; No significant cross-reactivity or interference between Poliovirus Receptor (PVR) and analogues was observed.
• Sequence Length: 392

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 6.0pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poliovirus Receptor (PVR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poliovirus Receptor (PVR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PVR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poliovirus Receptor (PVR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Poliovirus Receptor (PVR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poliovirus Receptor (PVR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Poliovirus Receptor (PVR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PVR elisa kit

Signal transduction; Tumor immunity; Infection immunity; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 7798736
• NCBI GeneID: 5817
• Molecular Weight: 42,864 Da
• NCBI Official Full Name: poliovirus receptor
• NCBI Official Synonym Full Names: poliovirus receptor
• NCBI Official Symbol: PVR
• NCBI Official Synonym Symbols: PVS; HVED; CD155; NECL5; TAGE4; Necl-5
• NCBI Protein Information: poliovirus receptor
• UniProt Protein Name: Poliovirus receptor
• Protein Family: Poliovirus receptor
• UniProt Gene Name: PVR
• UniProt Synonym Gene Names: PVS; NECL-5
• UniProt Entry Name: PVR_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

PVR: Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. Belongs to the nectin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Immunoglobulin superfamily; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; focal adhesion; cell surface; cytoplasm; plasma membrane; integral to membrane

Molecular Function: viral receptor activity; protein binding; receptor activity; cell adhesion molecule binding

Biological Process: intercellular junction assembly and maintenance; entry of virus into host cell; cell-cell adhesion; cell migration; regulation of immune response; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; response to virus; susceptibility to natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity