Rabbit MGP Polyclonal Antibody | anti-MGP antibody

Anti-MGP Antibody

Cat. Num. AAA178454

• Gene Names: MGP; NTI; GIG36; MGLAP
• Reactivity: Human; No cross reactivity with other proteins.
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: MGP; Polyclonal Antibody; Anti-MGP Antibody; GIG36; Matrix Gla protein; MGLAP; NTI; P08493; anti-MGP antibody

• Host: Rabbit
• Reactivity: Human; No cross reactivity with other proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 103

• Applicable Applications for anti-MGP antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot: 0.1-0.5mug/ml; Tested Species: Human; Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human; Tested Species:In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested.
• Immunogen: E Coli-derived human MGP recombinant protein (Position: Y20-F96). Human MGP shares 84.4% and 80.5% amino acid (aa) sequence identity with mouse and rat MGP, respectively.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of MGP expression in JURKAT whole cell lysates (lane 1). MGP at 12KD was detected using rabbit anti- MGP Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Immunohistochemistry (IHC)

(MGP was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- MGP Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Related Product Information for anti-MGP antibody

Rabbit IgG polyclonal antibody for Matrix Gla protein (MGP) detection.
Background: Matrix Gla protein (MGP) is an 84-residue vitamin K-dependent protein initially isolated from bovine bone. In addition, MGP is a 10-kD protein produced and secreted by vascular smooth muscle cells and chondrocytes and significantly accumulated in bone, cartilage, and dentin. It is also expressed at high levels in heart, kidney, and lung and is upregulated by vitamin D in bone cells. MGP has a high affinity binding to calcium ions, similar to other Gla-containing proteins. The protein acts as an inhibitor of vascular mineralization and plays a role in bone organization. And this gene is mapped to 12p12.3.

References

1. Hur, D. J., Raymond, G. V., Kahler, S. G., Riegert-Johnson, D. L., Cohen, B. A., Boyadjiev, S. A. A novel MGP mutation in a consanguineous family: review of the clinical and molecular characteristics of Keutel syndrome. Am. J. Med. Genet. 135A: 36-40, 2005.
2. Luo G, Ducy P, McKee MD, Pinero GJ, Loyer E, Behringer RR, Karsenty G (March 1997). "Spontaneous calcification of arteries and cartilage in mice lacking matrix GLA protein". Nature 386 (6620): 78-81.
3. Yao Y, Jumabay M, Ly A, Radparvar M, Cubberly MR, Boström KI (2013). "A role for the endothelium in vascular calcification". Circ. Res. 113 (5): 495-504.

NCBI and Uniprot Product Information

• NCBI GI #: 49574514
• NCBI GeneID: 4256
• NCBI Accession #: NP_000891.2
• NCBI GenBank Nucleotide #: NM_000900.3
• UniProt Accession #: P08493; Q2TU41; Q567P9; Q6ICN5; A0M8W5; B2R519; J3KMX7
• Molecular Weight: 15,325 Da
• NCBI Official Full Name: matrix Gla protein isoform 2
• NCBI Official Synonym Full Names: matrix Gla protein
• NCBI Official Symbol: MGP
• NCBI Official Synonym Symbols: NTI; GIG36; MGLAP
• NCBI Protein Information: matrix Gla protein
• UniProt Protein Name: Matrix Gla protein
• Protein Family: Matrix Gla protein
• UniProt Gene Name: MGP
• UniProt Synonym Gene Names: MGLAP; MGP
• UniProt Entry Name: MGP_HUMAN

NCBI Description

The protein encoded by this gene is secreted and likely acts as an inhibitor of bone formation. The encoded protein is found in the organic matrix of bone and cartilage. Defects in this gene are a cause of Keutel syndrome (KS). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

MGP: Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation. Defects in MGP are the cause of Keutel syndrome (KS). KS is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Secreted, signal peptide; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent; protein binding; structural constituent of bone

Biological Process: cartilage condensation

Disease: Keutel Syndrome