Western Blot (WB)
(Western blot detection of Eg5 in MCF7, 293T, Jurkat and Hela cell lysates using Eg5 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa.)
anti-Human Eg5 Monoclonal Antibody | anti-Eg5 antibody
Anti-Eg5 Mouse mAb
Gene Names
KIF11; EG5; HKSP; KNSL1; MCLMR; TRIP5
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry
Purity
Affinity Purified
Synonyms
Eg5; Monoclonal Antibody; Anti-Eg5 Mouse mAb; EG5; HKSP; KIF11; KIF11_HUMAN; kinesin family member 11; Kinesin like protein 1; Kinesin like spindle protein HKSP; Kinesin-like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; KNSL1; MCLMR; Thyroid receptor interacting protein 5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5; TRIP5; anti-Eg5 antibody
Host
Mouse
Source: Human
Source: Human
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4H3-1F12
Purity/Purification
Affinity Purified
Form/Format
Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1056
Applicable Applications for anti-Eg5 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
WB: 1:1000
ICC: 1:200
ICC: 1:200
Immunogen
Purified recombinant human Eg5 protein fragments expressed in E Coli.
Sensitivity
This antibody detects endogenous levels of Eg5 and does not cross-react with related proteins.
Sequence Similarities
Belongs to the kinesin-like protein family. BimC subfamily.
Contains 1 kinesin-motor domain.
Subunit Structure
Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Interacts (via C-terminus) with the kinase NEK6 in both interphase and mitosis.
Subcellular Location
Cytoplasm
Western Blot (WB)
(Western blot detection of Eg5 in MCF7, 293T, Jurkat and Hela cell lysates using Eg5 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa.)
Western Blot (WB)
(Western blot detection of Eg5 in MCF7, 293T, Jurkat and Hela cell lysates using Eg5 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa.)
Immunocytochemistry (ICC)
(Immunocytochemistry staining of Hela using Eg5 mouse mAb (1:200).)
Immunocytochemistry (ICC)
(Immunocytochemistry staining of Hela using Eg5 mouse mAb (1:200).)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Jurkat cell lysates using Eg5 mouse mAb.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Jurkat cell lysates using Eg5 mouse mAb.)
Related Product Information for anti-Eg5 antibody
Entrez Summary: This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
UniPort Summary Function: Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
UniPort Summary Function: Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
130
NCBI Official Full Name
kinesin-like protein KIF11
NCBI Official Synonym Full Names
kinesin family member 11
NCBI Official Symbol
KIF11
NCBI Official Synonym Symbols
EG5; HKSP; KNSL1; MCLMR; TRIP5
NCBI Protein Information
kinesin-like protein KIF11
UniProt Protein Name
Kinesin-like protein KIF11
UniProt Gene Name
KIF11
UniProt Synonym Gene Names
EG5; KNSL1; TRIP5; TR-interacting protein 5; TRIP-5
NCBI Description
This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq, Jul 2008]
Uniprot Description
Motor protein required for establishing a bipolar spindle during mitosis (PubMed:19001501). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769).
Research Articles on Eg5
Similar Products
Product Notes
The Eg5 kif11 (Catalog #AAA475058) is an Antibody produced from Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-Eg5 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Eg5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC). WB: 1:1000 ICC: 1:200. Researchers should empirically determine the suitability of the Eg5 kif11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Eg5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
