Testing Data
(1:500 dilution detected DYNEIN on 10ug of A431 lysates. A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with DYNEIN (1:500 dilution of this lot). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein DYNEIN (~74kD))
Mouse Dynein, Cytoplasmic, 74kD Intermediate Chains Monoclonal Antibody
Dynein, Cytoplasmic, 74kD Intermediate Chains
Reactivity
Bovine, Drosophila, Mouse, Rat, Sheep, Xenopus
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry
Purity
Affinity PurifiedPurified immunoglobulin
Synonyms
Dynein; Cytoplasmic; 74kD Intermediate Chains; Monoclonal Antibody; Anti -Dynein; anti-Dynein; 74kD Intermediate Chains antibody
Host
Mouse
Reactivity
Bovine, Drosophila, Mouse, Rat, Sheep, Xenopus
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2Q46
Specificity
Recognizes all known forms of the Mr 74kD intermediate chain subunit (IC74) of mammaliam cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the amino terminal portion of the molecule (a region conserved in both IC74 genes). Species Crossreactivity: Rat, mouse, bovine, sheep, fish, Drosophila and Xenopus. Does not react with squid.
Purity/Purification
Affinity Purified
Purified immunoglobulin
Purified immunoglobulin
Form/Format
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Applicable Applications for anti-Dynein, Cytoplasmic, 74kD Intermediate Chains antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Immunocytochemistry, Immunoblot and Immunoprecipitation.
Dilution: Immunocytochemistry: 1:50-1:100. Reacts with cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells.
Immunoprecipitation: 10ul/0.5g tissue. The monoclonal clearly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates.
Dilution: Immunocytochemistry: 1:50-1:100. Reacts with cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells.
Immunoprecipitation: 10ul/0.5g tissue. The monoclonal clearly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates.
Immunogen
Bovine brain cytoplasmic dynein
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(1:500 dilution detected DYNEIN on 10ug of A431 lysates. A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with DYNEIN (1:500 dilution of this lot). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein DYNEIN (~74kD))
Testing Data
(1:500 dilution detected DYNEIN on 10ug of A431 lysates. A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with DYNEIN (1:500 dilution of this lot). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein DYNEIN (~74kD))
Product Categories/Family for anti-Dynein, Cytoplasmic, 74kD Intermediate Chains antibody
Similar Products
Product Notes
The Dynein, Cytoplasmic, 74kD Intermediate Chains (Catalog #AAA607500) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Dynein, Cytoplasmic, 74kD Intermediate Chains reacts with Bovine, Drosophila, Mouse, Rat, Sheep, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Dynein, Cytoplasmic, 74kD Intermediate Chains can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC). Suitable for use in Immunocytochemistry, Immunoblot and Immunoprecipitation. Dilution: Immunocytochemistry: 1:50-1:100. Reacts with cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells. Immunoprecipitation: 10ul/0.5g tissue. The monoclonal clearly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates. Researchers should empirically determine the suitability of the Dynein, Cytoplasmic, 74kD Intermediate Chains for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dynein, Cytoplasmic, 74kD Intermediate Chains, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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