Testing Data
(1:500 dilution detected DYNEIN on 10ug of A431 lysates. A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with DYNEIN (1:500 dilution of this lot). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein DYNEIN (~74kD))
Mouse Dynein Monoclonal Antibody | anti-DYNC1I1 antibody
Dynein, Cytoplasmic, 74kD Intermediate Chains (HRP)
Gene Names
DYNC1I1; DH IC-1
Reactivity
Bovine, Drosophila, Mouse, Rat, Sheep, Xenopus
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
Dynein; Monoclonal Antibody; Cytoplasmic; 74kD Intermediate Chains (HRP); anti-DYNC1I1 antibody
Host
Mouse
Reactivity
Bovine, Drosophila, Mouse, Rat, Sheep, Xenopus
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2Q46
Specificity
Recognizes all known forms of the Mr 74kD intermediate chain subunit (IC74) of mammaliam cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the amino terminal portion of the molecule (a region conserved in both IC74 genes). Species Crossreactivity: Rat, mouse, bovine, sheep, fish, Drosophila and Xenopus. Does not react with squid.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-DYNC1I1 antibody
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)
Immunogen
Bovine brain cytoplasmic dynein
Conjugate
HRP
Preparation and Storage
Store at -20 degree C.
Testing Data
(1:500 dilution detected DYNEIN on 10ug of A431 lysates. A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with DYNEIN (1:500 dilution of this lot). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein DYNEIN (~74kD))
Testing Data
(1:500 dilution detected DYNEIN on 10ug of A431 lysates. A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with DYNEIN (1:500 dilution of this lot). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein DYNEIN (~74kD))
Product Categories/Family for anti-DYNC1I1 antibody
References
1. Journal of Cell Biology 127: 1671-1682 (1994). 2. PNAS USA 93: 141-144 (1996). 3. J. Biological Chemistry 271: 1687-1694 (1996). 4. Mol. Biology Cell 7: 331-343 (1996). 5. Journal of Cell Biology 118: 1133-1143 (1992). 6. Tai, A.W., et al., Cell 97: 877-887 (1999). 7. Reese, E.L. and Haimo, L.T., J. Cell Biology 151: 155-165 (2000). 8. Duncan, J.E. and Warrior, R., Current Biology 12: 1982-1991 (2002). 9. Hafezparast, M., et al., Science 300: 808-812 (2003). 10. Okuno, S., et al., J. Neuroscience 24: 7879-7887 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,556 Da
NCBI Official Full Name
cytoplasmic dynein 1 intermediate chain 1
NCBI Official Synonym Full Names
dynein, cytoplasmic 1, intermediate chain 1<
NCBI Official Symbol
DYNC1I1
NCBI Official Synonym Symbols
DH IC-1
NCBI Protein Information
cytoplasmic dynein 1 intermediate chain 1; dynein intermediate chain 1, cytosolic; cytoplasmic dynein intermediate chain 1
UniProt Protein Name
Cytoplasmic dynein 1 intermediate chain 1
Protein Family
UniProt Gene Name
DYNC1I1
UniProt Synonym Gene Names
DH IC-1
UniProt Entry Name
DC1I1_BOVIN
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Product Notes
The DYNC1I1 dync1i1 (Catalog #AAA6243796) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Dynein, Cytoplasmic, 74kD Intermediate Chains (HRP) reacts with Bovine, Drosophila, Mouse, Rat, Sheep, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Dynein can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the DYNC1I1 dync1i1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dynein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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