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Western Blot (WB) (Figure 1. Western blot analysis using Dynamin1 mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).)

Mouse anti-Human Dynamin-1 Antibody | anti-DNM1 antibody

Dynamin-1 Antibody

Gene Names
DNM1; DNM; EIEE31
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
Dynamin-1; Dynamin-1 Antibody; ECK; EPHA2; anti-DNM1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Isotype
IgG2a
Clone Number
3G4B6
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
851
Applicable Applications for anti-DNM1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of human Dynamin-1 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C

Western Blot (WB)

(Figure 1. Western blot analysis using Dynamin1 mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).)

Western Blot (WB) (Figure 1. Western blot analysis using Dynamin1 mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).)

Immunohistochemistry (IHC)

(Figure 2. Immunohistochemical analysis of paraffin-embedded human lymph tissue (A), glioma tissue (B) and cerebellum tissue (C), showing membrane localization using Dynamin1 mouse mAb with DAB staining)

Immunohistochemistry (IHC) (Figure 2. Immunohistochemical analysis of paraffin-embedded human lymph tissue (A), glioma tissue (B) and cerebellum tissue (C), showing membrane localization using Dynamin1 mouse mAb with DAB staining)
Related Product Information for anti-DNM1 antibody
Dynamin-1 (Dyn1), with 864-amino acid protein (about 95kDa), belongs to the dynamin family. Dynamin-1 (neuron-specific), dynamin-2 (ubiquitously expressed), and dynamin-3 (expressed only in the testis, brain, and lung), constitute the dynamin family. Members of the dynamin family are GPTase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin-1 is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C. Dynamin-1 is a large GTPase enzyme required in membrane constriction and fission during multiple forms of endocytosis. Dynamin-1 is also a key molecule required for the recycling of synaptic vesicles in neurons, and it has been known that dynamin-1 gene expression is induced during neuronal differentiation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
95,895 Da
NCBI Official Full Name
dynamin-1 isoform 2
NCBI Official Synonym Full Names
dynamin 1
NCBI Official Symbol
DNM1
NCBI Official Synonym Symbols
DNM; EIEE31
NCBI Protein Information
dynamin-1
UniProt Protein Name
Dynamin-1
Protein Family
UniProt Gene Name
DNM1
UniProt Synonym Gene Names
DNM

NCBI Description

This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.

Research Articles on DNM1

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Product Notes

The DNM1 dnm1 (Catalog #AAA850741) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Dynamin-1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Dynamin-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the DNM1 dnm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dynamin-1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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