Immunofluorescence (IF)
(Immunofluorescence staining of fixed cells infected with Dengue 2 Virus with anti-dsRNA antibody 1D3 (MBS488571) Immunofluorescence analysis of paraformaldehyde-fixed, triton-permeabilized cells infected with Dengue 2 virus stained with the chimeric rabbit IgG version of 1D3 () at 1/300 followed by Alexa 568 secondary antibody (red). The image is courtesy of Cecilia Alejandra Vazquez (IQUIBICEN (UBA-CONICET)).)
Rabbit anti-Virus dsRNA Monoclonal Antibody | anti-dsRNA antibody
Anti-dsRNA [1D3]
Reactivity
Virus
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified antibody. Protein A affinity purified
Synonyms
dsRNA; Monoclonal Antibody; Anti-dsRNA [1D3]; double-stranded RNA; double-stranded ribonucleic acid; anti-dsRNA antibody
Host
Rabbit
Reactivity
Virus
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
1D3
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-dsRNA antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
The antibody binds specifically to ds-RNA, which is part of the genetic material of some viruses, such as rotaviruses and the bluetongue virus. The antibody can be used as a test tool, to determine the nature of an unknown pathogen, and could potentially be used in gene therapy.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse format, for improved compatibility with existing reagents, assays and techniques.
PMID
918970
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed cells infected with Dengue 2 Virus with anti-dsRNA antibody 1D3 (MBS488571) Immunofluorescence analysis of paraformaldehyde-fixed, triton-permeabilized cells infected with Dengue 2 virus stained with the chimeric rabbit IgG version of 1D3 () at 1/300 followed by Alexa 568 secondary antibody (red). The image is courtesy of Cecilia Alejandra Vazquez (IQUIBICEN (UBA-CONICET)).)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed cells infected with Dengue 2 Virus with anti-dsRNA antibody 1D3 (MBS488571) Immunofluorescence analysis of paraformaldehyde-fixed, triton-permeabilized cells infected with Dengue 2 virus stained with the chimeric rabbit IgG version of 1D3 () at 1/300 followed by Alexa 568 secondary antibody (red). The image is courtesy of Cecilia Alejandra Vazquez (IQUIBICEN (UBA-CONICET)).)
Immunofluorescence (IF)
(Immunofluorescence staining of Zika virus-infected A549 cells with anti-dsRNA antibody 1D3 (MBS488571) Immunofluorescence analysis of Zika virus-infected A549 cells stained with the chimeric rabbit IgG version of 1D3 () followed by a secondary antibody. The nuclear stain is DAPI (blue). Panels show from left-right: (green) with DAPI and merged channels ( (green), DAPI (blue), Zika's E protein (red)). Images are courtesy of Cecilia Alejandra Vazquez (IQUIBICEN (UBA-CONICET)).)
Immunofluorescence (IF)
(Immunofluorescence staining of Zika virus-infected A549 cells with anti-dsRNA antibody 1D3 (MBS488571) Immunofluorescence analysis of Zika virus-infected A549 cells stained with the chimeric rabbit IgG version of 1D3 () followed by a secondary antibody. The nuclear stain is DAPI (blue). Panels show from left-right: (green) with DAPI and merged channels ( (green), DAPI (blue), Zika's E protein (red)). Images are courtesy of Cecilia Alejandra Vazquez (IQUIBICEN (UBA-CONICET)).)
Related Product Information for anti-dsRNA antibody
Recombinant monoclonal antibody to dsRNA. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1D3.
RDV-RNA-methylated bovine serum albumin complex.
RDV-RNA-methylated bovine serum albumin complex.
Product Categories/Family for anti-dsRNA antibody
References
Kitagawa et al. Immunogenicity of rice dwarf virus-ribonucleic acid. Tohoku J. Exp. Med. 122 (4), 337-343 (1977)
Note on publication: Describes the production and characterisation of antibodies against double-stranded RNA found in the rice dwarf virus (RDV). the antibodies are produced in rabbits immunized with anti-RDV-RNA-methylated bovine serum albumin complexes.
Note on publication: Describes the production and characterisation of antibodies against double-stranded RNA found in the rice dwarf virus (RDV). the antibodies are produced in rabbits immunized with anti-RDV-RNA-methylated bovine serum albumin complexes.
Similar Products
Product Notes
The dsRNA (Catalog #AAA488571) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-dsRNA [1D3] reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's dsRNA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). The antibody binds specifically to ds-RNA, which is part of the genetic material of some viruses, such as rotaviruses and the bluetongue virus. The antibody can be used as a test tool, to determine the nature of an unknown pathogen, and could potentially be used in gene therapy. Researchers should empirically determine the suitability of the dsRNA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "dsRNA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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