Rabbit anti-Human BCL11A Polyclonal Antibody | anti-BCL11A antibody

BCL11A, CT (BCL11A, CTIP1, EVI9, KIAA1809, ZNF856, B Cell lymphoma/leukemia 11A, B Cell CLL/lymphoma 11A, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 protein homolog, Zinc finger protein 856) (Azide free) (HRP)

Cat. Num. AAA6277893

• Gene Names: BCL11A; EVI9; CTIP1; ZNF856; HBFQTL5; BCL11A-L; BCL11A-S; BCL11a-M; BCL11A-XL
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: BCL11A; Polyclonal Antibody; CT (BCL11A; CTIP1; EVI9; KIAA1809; ZNF856; B Cell lymphoma/leukemia 11A; B Cell CLL/lymphoma 11A; COUP-TF-interacting protein 1; Ecotropic viral integration site 9 protein homolog; Zinc finger protein 856) (Azide free) (HRP); B-cell lymphoma/leukemia 11A; B-cell CLL/lymphoma 11A; Zinc finger protein 856; anti-BCL11A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BCL11A.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BCL11A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 806-835 in the C-terminal region of human BCL11A, UniProt Accession #Q9H165.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat, Ramos, 293, NCI-H460 cell line lysates (35ug/lane) usingMBS6009008. BCL11A (arrow) was detected usingMBS6009008.)

Related Product Information for anti-BCL11A antibody

BCL11A is a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6.

Product Categories/Family for anti-BCL11A antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Solovieff, N., et al. Blood 115(9):1815-1822(2010). 2. Nuinoon, M., et al. Hum. Genet. 127(3):303-314(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 20336307
• NCBI GeneID: 53335
• NCBI Accession #: NP_060484.2
• NCBI GenBank Nucleotide #: NM_018014.3
• UniProt Accession #: Q9H165; Q86W14; Q8WU92; Q96JL6; Q9H163; Q9H164; Q9H3G9; Q9NWA7; D6W5D7
• Molecular Weight: 91,197 Da
• NCBI Official Full Name: B-cell lymphoma/leukemia 11A isoform 2
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 11A (zinc finger protein)
• NCBI Official Symbol: BCL11A
• NCBI Official Synonym Symbols: EVI9; CTIP1; ZNF856; HBFQTL5; BCL11A-L; BCL11A-S; BCL11a-M; BCL11A-XL
• NCBI Protein Information: B-cell lymphoma/leukemia 11A; EVI-9; BCL-11A; zinc finger protein 856; C2H2-type zinc finger protein; COUP-TF-interacting protein 1; ecotropic viral integration site 9 homolog; ecotropic viral integration site 9 protein homolog; B-cell CLL/lymphoma 11A (z
• UniProt Protein Name: B-cell lymphoma/leukemia 11A
• Protein Family: B-cell lymphoma/leukemia
• UniProt Gene Name: BCL11A
• UniProt Synonym Gene Names: CTIP1; EVI9; KIAA1809; ZNF856; BCL-11A; EVI-9
• UniProt Entry Name: BC11A_HUMAN

NCBI Description

This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Bcl-11A: Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1. Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; C2H2-type zinc finger protein; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 2p16.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein homodimerization activity; protein heterodimerization activity; metal ion binding; transcription corepressor activity

Biological Process: protein sumoylation; transcription, DNA-dependent; B cell differentiation; regulation of dendrite development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of collateral sprouting; negative regulation of transcription from RNA polymerase II promoter; negative regulation of axon extension; positive regulation of collateral sprouting; negative regulation of protein homooligomerization; T cell differentiation