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Sequence Information

Calcium/Calmodulin Dependent Protein Kinase I Recombinant Protein | CAMK1 recombinant protein

Recombinant Calcium/Calmodulin Dependent Protein Kinase I (CAMK1)

Gene Names
CAMK1; CAMKI
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
Calcium/Calmodulin Dependent Protein Kinase I; Recombinant Calcium/Calmodulin Dependent Protein Kinase I (CAMK1); CAMKI; CaM kinase I alpha; CAMK1 recombinant protein
Ordering
For Research Use Only!
Host
E.coli
Purity/Purification
> 90%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01%skl, 5% Trehalose.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Applicable Applications for CAMK1 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western BLot (WB).
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Met1~Leu370
Tags
N-terminal His Tag
Subcellular Location
Cytoplasm
Traits
Freeze-dried powder
Predicted isoelectric point
5.1
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

Sequence Information

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 45.0kDa
Accurate Molecular Mass: 45kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
calcium/calmodulin-dependent protein kinase type 1
NCBI Official Synonym Full Names
calcium/calmodulin dependent protein kinase I
NCBI Official Symbol
CAMK1
NCBI Official Synonym Symbols
CAMKI
NCBI Protein Information
calcium/calmodulin-dependent protein kinase type 1
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type 1
UniProt Gene Name
CAMK1
UniProt Synonym Gene Names
CaM-KI; CaMKI-alpha
UniProt Entry Name
KCC1A_HUMAN

NCBI Description

Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMK1A: an ubiquitous protein kinase of the CAMK1 family. Activated by Ca(2+)/calmodulin. Must be phosphorylated to be maximally active. Substrates include synapsin I.

Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.17; Kinase, protein; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytoplasm; nucleus

Molecular Function: ATP binding; calmodulin binding; calmodulin-dependent protein kinase activity; protein binding

Biological Process: cell cycle; negative regulation of protein binding; nucleocytoplasmic transport; positive regulation of muscle cell differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein export from nucleus; positive regulation of synapse structural plasticity; positive regulation of syncytium formation by plasma membrane fusion; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of muscle cell differentiation; regulation of protein binding; regulation of protein localization; signal transduction

Research Articles on CAMK1

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Product Notes

The CAMK1 camk1 (Catalog #AAA2034255) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Calcium/Calmodulin Dependent Protein Kinase I can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western BLot (WB). Researchers should empirically determine the suitability of the CAMK1 camk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calcium/Calmodulin Dependent Protein Kinase I, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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