Mouse anti-Human, Mouse DNMT3L Monoclonal Antibody | anti-DNMT3L antibody

DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like MGC1090)

Cat. Num. AAA608699

• Gene Names: DNMT3L; MGC1090
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: DNMT3L; Monoclonal Antibody; DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like MGC1090); Anti -DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like MGC1090); anti-DNMT3L antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10F698
• Specificity: Recognizes human DNMT3. No Crossreactivity: against DNMT3a or DNMT3B.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH7.4, 50% glycerol and 0.09% sodium azide.

• Applicable Applications for anti-DNMT3L antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: Dilution: Western Blot:1-10ug/ml; Immunocytochemistry:0.1-1ug/ml
• Immunogen: Fusion protein aa 1-387 of human DNMT3L (also known as DNA (cytosine-5)methyltranferase 3-like, accession number BAA95556).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-DNMT3L antibody

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue (1-6). Studies show that DNMT3L regulates the activity of DNMT3A and DNMT3B and stimulates their catalytic activities (7). DNMT3L has specifically been linked to the process of carcinogenesis, thru its role in nuclear

Product Categories/Family for anti-DNMT3L antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 28872780
• NCBI GeneID: 29947
• NCBI Accession #: NP_787063.1
• NCBI GenBank Nucleotide #: NM_175867.1
• UniProt Accession #: Q9UJW3; Q9BUJ4; E9PB42
• Molecular Weight: 43,583 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3-like isoform 2
• NCBI Official Synonym Full Names: DNA (cytosine-5-)-methyltransferase 3-like
• NCBI Official Symbol: DNMT3L
• NCBI Official Synonym Symbols: MGC1090
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3-like; DNA (cytosine-5)-methyltransferase 3-like; OTTHUMP00000109516; OTTHUMP00000109517; cytosine-5-methyltransferase 3-like protein; human cytosine-5-methyltransferase 3-like protein
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3-like
• Protein Family: DNA (cytosine-5)-methyltransferase
• UniProt Gene Name: DNMT3L
• UniProt Entry Name: DNM3L_HUMAN

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined. [provided by RefSeq]

Uniprot Description

DNMT3L: Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. Belongs to the C5-methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleus; cytosol

Molecular Function: protein binding; enzyme binding; metal ion binding; enzyme activator activity

Biological Process: positive regulation of catalytic activity; genetic imprinting; DNA methylation; spermatogenesis; gene expression; negative regulation of transcription, DNA-dependent; regulation of gene expression, epigenetic; DNA methylation on cytosine